山羊绒生长周期差异的比较转录组研究

项目名称： 山羊绒生长周期差异的比较转录组研究

项目编号： No.31272421

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 李金泉

作者单位： 内蒙古农业大学

项目金额： 70万元

中文摘要： 转录组研究能够从整体水平研究基因功能以及基因结构，揭示特定生物学过程以及疾病发生过程中的分子机理。基于近年来比较转录组的研究进展，本研究选取埋植褪黑素和自然生长条件下，山羊绒不同发育阶段（生长期、休止期和退行期）的皮肤为样本，进行转录组测序，建立山羊皮肤组织基因比较转录数据库，发掘山羊绒周期的特异基因；对差异基因通过将转录本映射到基因组，初步绘制山羊绒周期发育相关基因的基因组分布图；通过GO功能分析和KEGG Pathway分析，探索差异基因对山羊绒周期的调控通路和机制；并通过荧光定量和组织杂交，鉴定周期特异的分子标记及其表达的部位。本项目对毛囊发育相关基因的深入研究旨在为人工调控绒毛生长方案的制定提供依据，也为绒山羊分子育种奠定基础。

中文关键词： 山羊绒；调控网络；比较转录组；启动机制；褪黑素

英文摘要： Analysis of transcriptome can be widely used to analyze global patterns of gene function and structure, providing researchers with greater insights into biological pathways and molecular mechanisms that regulate disease progression. In this study, the skin samples which are implanted melatonin and under natural growth conditions respectively from Inner Mongolian cashmere goats in during the growth (anagen), involution (catagen), and rest (telogen) phases of hair growth were collected to proforme transcriptome sequencing, to create a transcription database of gene in goat skin, and to identify specific genes in cashmere growth cycle. Gene maps associated with cashmere cycle development are drawn by mapping transcripts to the genome. Differently expressed genes involved in the regulatory pathways and mechanisms are explored by GO functional analysis and the KEGG Pathway analysis. The cycle-specific molecular markers and their expression site are identified by hybridization of fluorescent quantitative and organizations. The project aims to provide a basis for developing the program of artificially regulating hair growth, and to make a solid foundation for future cashmere molecular breeding.

英文关键词： Cashmere；Regulatory network；Comparative transcriptional；Initiation mechanism；Melatonin