极低频电磁场对脑缺血大鼠内源性神经干细胞增殖和分化的影响及机制研究

项目名称： 极低频电磁场对脑缺血大鼠内源性神经干细胞增殖和分化的影响及机制研究

项目编号： No.81201513

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学四处

项目作者： 高强

作者单位： 四川大学

项目金额： 24万元

中文摘要： 脑缺血等损伤可激活内源性神经干细胞增殖和分化，这种增殖和分化与Notch信号通路有密切的关系，但增殖产生的细胞数目较少不足以完成自身修复，仅有极少量的坏死神经元被新生神经元替代。 最近研究证明，极低频电磁场（ELF-EMF）可以增强体外培养细胞或正常实验动物神经突触的活动、促进神经干细胞分化和神经再生。本课题试行将ELF-EMF应用于脑缺血研究，观察ELF-EMF对脑缺血大鼠不同时间点的神经功能的影响、大鼠海马齿状回颗粒下层（SGZ）和室管膜下区（SVZ）以及缺血半影区的NICD蛋白和Hes1、Hes5基因表达的情况以及内源性神经干细胞增殖和分化的情况，以探索ELF-EMF是否通过Notch信号通路对脑缺血大鼠内源性神经干细胞的增殖和分化产生影响，并揭示其随脑缺血后时间变化的规律。

中文关键词： 极低频电磁场；脑缺血；神经干细胞；Notch信号通路；

英文摘要： Cerebral ischemia and other damage can activate the endogenous neural stem cell proliferation and differentiation which are closely related with Notch signaling pathway. But the cell proliferation g is insufficient to complete the repair itself, only a very small amount of necrotic neurons could be replaced by newborn neurons. Recent studies have shown that extremely low frequency electromagnetic fields (ELF-EMF) can enhance in vitro activity of synapses or promote neural stem cell differentiation and nerve regeneration in vitro or in normal rats. This subject are trying to apply the ELF-EMF in cerebral ischemia rats and observe the effect of the ELF-EMF on the neurological function at different time points, the expression of NICD protein, Hes1, Hes5 gene, the neural stem cell proliferation and differentiation in the regions of the dentate gyrus granular lower layer (SGZ), subventricular zone (SVZ) as well as the ischemic penumbra, to explore whether the ELF-EMF could effect the endogenous neural stem cells proliferation and differentiation through the Notch signaling pathway on rats with cerebral ischemia, and reveals its regulation with the time changes.

英文关键词： extremely low frequency electromagnetic fields；cerebral ischemia；neural stem cells；Notch signaling pathway；