基于Rho/Rho激酶通路研究针刺干预自发性高血压大鼠血管重塑平滑肌细胞增殖、迁移的作用及机制

项目名称： 基于Rho/Rho激酶通路研究针刺干预自发性高血压大鼠血管重塑平滑肌细胞增殖、迁移的作用及机制

项目编号： No.81503635

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 史慧妍

作者单位： 天津中医药大学

项目金额： 18万元

中文摘要： 血管重塑是高血压病的病理变化，也是高血压病导致靶器官损害的结构基础。颈总动脉内膜中层厚度改变已成为预测脑卒中一项重要指标。Rho/Rho激酶通路参与高血压病的发生、发展并影响细胞粘附与迁移、平滑肌收缩凋亡、细胞增殖等生物行为。抑制该通路可取得显著内皮保护作用。eNOS是该通路重要效应分子。针刺既能调控血压又具有保护靶器官作用。前期研究显示针刺能改善患者血压昼夜节律，基础研究提示针刺有降低AngⅡ趋势。基于此，本研究应用自发性高血压大鼠,以厄贝沙坦作为对照，利用RT-PCR、Western blot、ELISA、免疫组化、放免法、硝酸还原酶法、比色法、形态学等，研究针刺对Rho/Rho激酶通路的影响，干预血管内皮细胞增殖、迁移的作用以及颈动脉血管形态改变。探讨针刺对高血压靶器官保护作用及Rho/Rho激酶通路作用。

中文关键词： 高血压；针刺；血管重塑；Rho/Rho激酶

英文摘要： Vascular remodeling is not only the pathology base of hypertension, but also the structural basis of target-organ damage.Carotid artery remodeling has become an important index in prediction of the stroke.Rho / Rho-kinase pathway is involved in the occurrence of hypertension and development. Involved in cell adhesion and migration, apoptosis of smooth muscle contraction, cell proliferation and other biological behavior.NO plays a key role in vasodilation dysfunction of endothelium-dependent,and plays an important regulation to endothelial cell proliferation and inflammation.The latest study shows Rho / Rho kinase pathway is a new target in hypertension, inhibition of this pathway can protect endothelial significantly, eNOS is an important effector molecules of this pathway. Acupuncture can Improve the circadian rhythm of blood pressure,reduce AngII.applying spontaneously hypertensive rats (SHR), using theRT-PCR, the western blot ,ELISA,Immunohistochemistry, radiation immunology ,Nitrate reductase ,colorimetry and morphology methods to study the influence of the acupuncture to blood pressure and vascular remodeling, and discuss the effect of acupuncture to hypertension, the protection of target organs and the function of Rho / Rho kinase pathway.

英文关键词： Hypertension;acupuncture;vascular remodeling;Rho/Rho-kinase