基于微液滴的高通量多元分析新方法及肿瘤标志物的分析检测

项目名称： 基于微液滴的高通量多元分析新方法及肿瘤标志物的分析检测

项目编号： No.21205089

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 分析化学

项目作者： 吉邢虎

作者单位： 武汉大学

项目金额： 25万元

中文摘要： 本项目开展基于微液滴的高通量多元分析新方法及肿瘤标志物分析检测的基础性研究。液滴微流控芯片以皮升级体积、kHz频率的单分散性微液滴作为操作单元，为高通量筛选提供了有效技术平台；同时，以固定体积的微液滴作为反应和检测单元，分析检测信号可在液滴内随时间不断积累，有利于提高分析检测的灵敏度，为液滴内超痕量分析对象的高灵敏检测提供了新方法；基于量子点的光学编码微球在多元分析应用中优势独特，在液滴微流控芯片中，以单个微液滴对单个量子点光学编码微球进行包裹，借助生物功能化的量子点光学编码微球的编码标记以及生物识别功能，实现液滴内目标对象的识别与分析，是一种多元分析的新思路。上述功能的微流控芯片集成化，可建立一种基于微液滴的高灵敏、高通量、多元分析新方法。该方法用于肝癌肿瘤标志物的多元分析检测，为多组份肿瘤标志物联合应用在提高肝癌早期诊断的准确性、指导癌症治疗等方面提供基础性技术支持。

中文关键词： 微流控芯片；微液滴；高通量；多元分析；肿瘤标志物

英文摘要： This project focuses on droplet microfluidics for high-throughput and multiplex assay of tumor markers. Droplet microfluidics focuses on creating discrete volumes with the use of immiscible phases, producing highly monodisperse droplets in the femtoliter to nanoliter range, at rates of up to kHz.Such rate and volumes offer significant advantages for performing high-throughput screens and assays. Furthermore, discrete microdroplets act as reaction and detection containers, with the target signals remaining confined inside each droplet and increasing in intensity over time, which permits amplified detection of extremely low levels of target molecules. In addition, the spectral coding technology based on quantumn dot-encoded microbeads offer a potential platform for multiplexed bioassays. Thus an optically-coded droplet strategy based on the encapsulation of quantum-dot-tagged microbeads into microdroplets is conceived for the identification of the droplets composition. Using the optical coding scheme, the target molecules in microdroplets can be recognized with biomolecule probes on the surface of the barcoded microbeads, which have relations with the quantum-dot fluorescent spectral codes. Based on these functions, a novel droplet microfluidic platform is developed for high-throughput and multiplex assay of tumor

英文关键词： Microfluidics；Droplet；High-throughput；Multiplex assay；Tumor marjer