糖基化修饰大豆蛋白过敏原的调控机制研究

项目名称： 糖基化修饰大豆蛋白过敏原的调控机制研究

项目编号： No.31201293

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 布冠好

作者单位： 河南工业大学

项目金额： 24万元

中文摘要： 大豆蛋白是较易引起过敏的食物之一，目前还没有有效的方法来消除大豆蛋白过敏性。通过糖基化修饰对大豆蛋白过敏原进行调控，以期建立有效控制大豆蛋白过敏原的方法。首先通过糖基化反应制备大豆蛋白-糖复合物，采用酶联免疫吸附分析方法和动物免疫学实验检测复合物中主要过敏蛋白的抗原性和过敏特性，确定具有较低抗原性和过敏特性的糖基化反应条件和糖基供体。利用生物质谱方法（MALDI-TOF-MS）分析大豆蛋白糖复合物中糖基化反应的位点和糖蛋白的修饰度，SDS-PAGE、SEC、DSC、CD光谱、傅里叶红外光谱（FTIR）等测定糖蛋白的分子组成特性及结构变化规律，分析大豆蛋白糖复合物中主要过敏蛋白的分子组成与其抗原性及过敏特性的相关性，建立有效控制大豆蛋白过敏原的糖基化反应模式，揭示糖基掩蔽大豆蛋白过敏原的作用机理。这将为低敏性大豆蛋白制品的开发提供重要理论依据。

中文关键词： 大豆蛋白；糖基化；抗原性；过敏原性；结构特性

英文摘要： Soybean protein is one of food allergens, at present there is no effective method for eliminating the allergenicity of soybean protein. In this research, we employ glycosylated modification to regulate the soybean protein allergen in order to establish effective ways to control the allergens. The complex of soybean protein and sugar are firstly prepared by glycosylation. In the follwing, the antigenicity and allergenicity of main protein allergens in the complex are determined by enzyme-linked immunosorbent assay and animal immunology experiments, and glycosylation reaction conditions including glycosylation donors are determined to obtain the complex with reduced allergenic properties. The glycosylation reaction sites and the modification degree of soybean protein are detected by means of Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF-MS), molecular composition characteristics and the structure changes are measured by SDS-PAGE, Size-exclusion Chromatography (SEC), Differentical Scanning Calorimetry (DSC), Circular Dichroism Spectrum and Fourier Transform Infrared Spectroscopy (FTIR). Through analysing the correlation between molecular composition and antigenicity and allergenicity of glycosylated protein complex, a glycosylation model will be established, which can effec

英文关键词： Soybean protein；glycosylation；antigenicity；allergenicity；structural characteristics