LncRNA ecG0S2-G0S2甲基化调控途径在重症肌无力T淋巴细胞免疫平衡中的机制研究

项目名称： LncRNA ecG0S2-G0S2甲基化调控途径在重症肌无力T淋巴细胞免疫平衡中的机制研究

项目编号： No.81501034

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 徐立群

作者单位： 中南大学

项目金额： 17.5万元

中文摘要： T淋巴细胞亚群免疫功能失衡在重症肌无力（MG）的发病过程中起着重要作用。G0S2是淋巴细胞增殖的重要调控分子，我们发现，G0S2基因在重症肌无力患者外周血T淋巴细胞中的表达高度上调，其启动子区甲基化程度则显著下调。lncRNA调控基因甲基化在多种疾病的发生中具有重要作用，但在MG中的作用尚未报道。本项目拟从表观遗传学的角度，研究lncRNA ecG0S2通过甲基化转移酶干扰启动子区甲基化，从而影响G0S2表达的调控途径，进而阐明lncRNA ecG0S2-G0S2甲基化对T淋巴细胞免疫平衡的作用及MG发病的分子机制，为开发MG的表观治疗方略提供新的思路和理论依据。

中文关键词： 重症肌无力；长链非编码RNA；G0/G1期开关基因2；甲基化；甲基化转移酶

英文摘要： The dysregulation of T lymphocytes is of great importance in the pathogenesis of myasthenia gravis. G0S2 is a key regulator during lymphocyte proliferation. Our previous studies showed the increased expression of G0S2 in the peripheral T lymphocytes of MG patients. Meanwhile, DNA methylation level of G0S2 promoter is downregulated. Many evidences already proved how the lncRNA regulates gene expression by changing DNA methylation of target gene. However, it is still unknown if lncRNA is able to play the similar role during MG pathogenesis. Our future study aims to investigate the role of lncRNA ecG0S2 regarding how it can regulate G0S2 methylation through DNA methyltransferases and finally the expression of G0S2 gene, which may play a major role for maintaining the immune balance of T lymphocytes. Better understanding of this regulation process can provide more evidence to explain the molecular pathomechanism of MG, and offer new perspective on the epigenetic therapy of MG.

英文关键词： myasthenia gravis;long non-coding RNA;G0/G1 switch gene 2;methylation;DNA methyltransferase