镰形棘豆总黄酮干预炎症介导的胰岛素抵抗分子机制研究

项目名称： 镰形棘豆总黄酮干预炎症介导的胰岛素抵抗分子机制研究

项目编号： No.81202971

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 杨丽霞

作者单位： 甘肃省中医药研究院

项目金额： 23万元

中文摘要： 胰岛素抵抗（IR）被国际公认是2型糖尿病发病中的主要环节，也是高血压、高血脂等疾病的共同病理基础。因此，寻找有效改善IR的药物成为研究的热点。目前，许多研究发现，植物黄酮类化合物具有降糖、降脂、改善IR的作用。IR的形成机制复杂，至今其分子机制尚未完全阐明。近年来，越来越多的研究表明：慢性炎症与IR相关，并且是形成IR的原因。本研究所前期研究发现，镰形棘豆总黄酮具有抗炎、抑菌、抗氧化的药理作用，但作为黄酮类化合物在IR方面的研究尚未开展。因此，本课题拟从"炎症信号通路"与"胰岛素信号通路"相结合的角度出发，采用的KK-Ay小鼠这一国际公认的IR动物模型，以及细胞3T3-Ll IR细胞模型，利用现代分子生物学实验技术，从整体水平、细胞水平出发，全面探讨镰形棘豆总黄酮干预炎症介导的IR的作用靶点及分子机制，为其临床应用提供科学依据，为祖国医药防治IR的研究提供新思路和新方法。

中文关键词： 镰形棘豆总黄酮；炎症；胰岛素抵抗；信号通路；分子机制

英文摘要： Insulin resistance (IR) is internationally recognized as the key in the incidence of type 2 diabetes, but also the common pathological basis of the high blood pressure, high cholesterol and other diseases. Therefore, it is a research hotspot to find the effective drug in improving IR. Recently, many studies showed that flavonoids could hypoglycemic, antihyperlipidemics and improve IR. The IR mechanism is complex, up to the present its molecular mechanism has not yet been fully elucidated. In recent years, more and more research showed that the chronic inflammation is associated with IR, and it is also the reason of IR. A preliminary study of our Institute showed that the total flavonoids of Oxytropis Falcate Bunge have anti-inflammatory, antibacterial and antioxidant pharmacological effects, but not yet carried out in the IR as flavonoids. Therefore, this project intends to use KK-Ay mice which is an internationally recognized IR animal models, as well as 3T3-Ll cell which is the IR cell model, combining inflammatory signaling pathway and insulin signaling pathways, using modern molecular biology experimental techniques, from the overall level and cellular level to explore the effect targets and molecular mechanisms of the total flavonoids of Oxytropis Falcate Bunge on IR mediated by inflammation. And this study

英文关键词： the total flavonoids of Oxytropis Falcate Bunge；inflammation；insulin resistance；signaling pathway；molecular mechanisms