SiO2-抗生素纳米抗菌剂的制备及在细菌感染诊疗中的应用研究

项目名称： SiO2-抗生素纳米抗菌剂的制备及在细菌感染诊疗中的应用研究

项目编号： No.51303036

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 一般工业技术

项目作者： 李莉莉

作者单位： 国家纳米科学中心

项目金额： 25万元

中文摘要： 细菌感染是当前世界上对人类健康最具有威胁的疾病之一，迫切需要研发新型药物载体，以提高药物疗效、减少药物毒性和降低耐药风险。本项目将以具有荧光信号的介孔二氧化硅(MSNs)为主体材料，将固相合成法合成的万古霉素响应性多肽和甘露糖PEG2000以一步法共价偶联修饰在MSNs表面上，制备新型的具有细菌感染微环境响应性的SiO2-抗生素纳米抗菌剂。设计体内体外模型，阐明其响应性释放、抗菌性能、生物毒性、低耐药性及体内成像等特性。同时通过可控制备理化参数不同的纳米抗菌剂，揭示其理化参数与细菌感染微环境响应性的关系及抗菌机理。该项目的成功实施有望在推动细菌感染的诊疗一体化进程中取得突破性进展，拟为体内细菌感染的诊断和治疗提供了新的纳米载药体系和新的研究思路。

中文关键词： 细菌感染；抗生素耐药；巨噬细胞；微环境响应；活体成像

英文摘要： Currently, bacterial infection is one of the diseases which causing a major threat to human health in the world. It is an urgent need that a kind of novel drug carrier should be developed to improve the efficacy of drugs, reduce toxicity and the risk of drug-resistance. Mesoporous silica (MSNs), the project will have a fluorescent signal as the main material. This project will use MSNs which having fluorescent signal inside as the main material. And the surface of MSNs was modified by vancomycin coupled responsive peptide and mannose PEG2000 using one-step covalent modification to prepare a novel bacterial infection micro-environment responsive nano-antibacterial agent. In vitro and in vivo models were designed to clarify its micro-environment responsive release, biological toxicity, lower drug-resistance, infected site fluorescence imaging and so on. On the other hand, by preparing controllable of the nano-antibacterial agent, the relationship between its physicochemical parameters and bacterial infected micro-environment responsiveness and its anti-bacterial mechanism were revealed. It is expected that the succesful imlementation of the project will have a breakthrough in promoting the theranostic of bacterial infection.A breakthrough in the successful implementation of the project is expected to promote the i

英文关键词： Bacterial Infection；Antibiotic resistance；Macrophage；Microenvironment responsiveness；In vivo bioimaging