荧光稀土配位聚合物的制备及其靶向监测癌细胞内单线态氧的研究

项目名称： 荧光稀土配位聚合物的制备及其靶向监测癌细胞内单线态氧的研究

项目编号： No.21305054

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 谭宏亮

作者单位： 江西师范大学

项目金额： 25万元

中文摘要： 细胞内单线态氧的靶向监测对于揭示生物氧化衰老机理、正确评价光动力学治疗效果及其治疗过程中药物剂量的及时调整有着重要的科学意义和实用价值。纳米尺寸的配位聚合物（nanoscaled coordination polymers，NCPs），特别是稀土荧光NCPs，作为一种新型的荧光探针在检测细胞内生物分子方面备受青睐。本课题旨在没有客体发光配体参与的情况下，直接利用生物分子（鸟嘌呤和鸟苷酸）与稀土离子配位制备生物相容性好、发光效率高的NCPs，并以该NCPs为荧光探针通过时间分辨分析技术对癌细胞内的单线态氧进行靶向监测，以克服大多数有机荧光探针本身水溶性差、生物相容性不好以及易受背景荧光干扰等缺陷。同时，通过研究稀土荧光NCPs的形成机理和阐明NCPs的形状、尺寸等物理特征与其荧光性能和分析灵敏度之间的关系，为指导设计合成形貌可控、具有特定功能的NCPs及其在生物医学领域的应用提供理论依据。

中文关键词： 稀土配位聚合物；生物分子；荧光传感；单线态氧；生物医学应用

英文摘要： Target monitoring the level of singlet oxygen in cell is of great scientific and practical importance, because it is benefit to reveal the aging mechanism induced by biological oxidation, and to accurate evaluate the therapeutic efficacy of photodynamic therapy (PDT) and adjust the drug dose in the process of PDT. Nanoscaled coordination polymers (NCPs), particularly fluorescent lanthanide NCPs, have received increasing attention, due to their potential application as a novel fluorescent probe in the detection of biological molecules in cell with highly sensitivity. In this project, we attempt to prepare a series of fluorescent lanthanide NCPs by directly using guanine and its nucleotides as bridge ligands. Auxiliary ligands that can sensitize the fluorescence of lanthanide ions were not added during the synthetic process of NCPs. These fluorescent lanthanide NCPs with excellent biocompatibility and highly emission efficiency were further used to tagert monitor the level of singlet oxygen in cancer cell. More important, the shortcomings of organic fluorescent probe, such as poor water soluble and biocompatible, and tend to be interfered by background fluorescence, can be overcome by using lanthanide NCPs to detect singlet oxygen in cancer cell in the mode of time-resolved fluorescence. Meanwhile, we will investi

英文关键词： Lanthanide coordination polymer；Biological molecules；Fluorescent sensing；Singlet oxygen；Biomedical application