结核分枝杆菌致病相关蛋白质-RNA二元网络构建及功能研究

项目名称： 结核分枝杆菌致病相关蛋白质-RNA二元网络构建及功能研究

项目编号： No.U1401224

项目类型： 联合基金项目

立项/批准年度： 2015

项目学科： 管理科学

项目作者： 毕利军

作者单位： 中国科学院生物物理研究所

项目金额： 243万元

中文摘要： 结核病由结核分枝杆菌感染所致，曾因抗生素的发明得到有效控制。但随着耐药菌的出现及与HIV共感染等问题使结核病又死灰复燃。结核分枝杆菌致病过程可以归因于病原所携带的因子与宿主的组织细胞成分相互作用的结果。起致病的因子可能较多，还可能有深层次的分子调控网络。我们最近研究表明：（1）新发现一些蛋白质能引起宿主的免疫反应，这些可能与结核分枝杆菌的致病性相关；（2）新发现结核分枝杆菌非编码RNA与致病过程相联系。我们将构建包括致病相关蛋白质、非编码RNA等关键成分组成的互作网络并开展关键网络节点蛋白质及复合物的功能性研究，寻找未知的结核分枝杆菌致病因子及其调控机制。这些分子信息与临床信息的结合，将十分有助于了解结核分枝杆菌深层次致病机制。

中文关键词： 结核病；非编码RNA；致病；二元网络

英文摘要： The infection of Mycobacterium tuberculosis leads to tuberculosis (TB), which was controlled with the application of antibiotics. However, TB remains a global health problem compounded by human immunodeficiency virus (HIV) co-infection and drug resistant bacteria. Tuberculosis pathogenesis is the result of the interaction between the bacterial virulence and the cellular component from the host. Many factors involved in this process and a complex signal transduction and regulation network might contribute to it as well. Our recent research revealed: (1) novel discovered protein could provoke the host immune response which might related to the TB pathogenesis; (2) novel discovered none-coding RNAs are correlated to the TB pathogenesis. We are going to establish an interaction network including the key components such as pathogenic proteins and none-coding RNAs. Therefore, we could study the function of those proteins/complexes at the key node of this network; look for the unknown TBpathogenic factors and understand their regulatory mechanisms. The integration of information from both research and clinic side would provide a good opportunity to understand the TB pathogenesis mechanisms in deep.

英文关键词： tuberculosis;none coding RNA;pathogenesis;two component network