乳酪乳清有效部位分离提取及其有关PPARs的抗动脉粥样硬化机制研究

项目名称： 乳酪乳清有效部位分离提取及其有关PPARs的抗动脉粥样硬化机制研究

项目编号： No.30860335

项目类型： 地区科学基金项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 新华·那比

作者单位： 新疆医科大学

项目金额： 25万元

中文摘要： 新疆传统发酵乳酪乳清（简称TFCW）在哈萨克族民间长期用于调节血脂、血糖。本课题组研究发现TFCW具有抗实验性动脉粥样硬化作用。为确立其有效部位和作用机制，本研究首先对其中发酵菌进行分离纯化,然后结合形态学、生理生化特性和16SrDNA、16SrRNA、26SrDNAD1/D2区基因序列分析方法进行鉴定。确定TFCW中含有两种有价值的微生物：乳酸菌和酵母菌；归属：短乳杆菌、植物乳杆菌、瑞士乳杆菌、马乳酒样乳杆菌、干酪乳杆菌和香肠乳杆菌、乳酸乳球菌；单孢酿酒酵母和东方伊萨酵母。其次对TFCW进行提取分离，并确定分子量在1071-724Da肽段对ACE抑制率达56.45%；分子量分布在645-500Da肽段对自由基DPPHo、oOH、O2-o抑制率是59.06%、62.0%。第三通过利用高血脂大鼠模型，确定TFCW的机制与调血脂，升高LCAT的活性，增加NO含量，增强oOH的清除能力以及增强HDL水平与其清道夫SR-BI受体相互作用，显著降低CRP、富含单不饱和脂肪酸、丝氨酸和镁、钙、钾等元素作用有关。此外机制中与PPARs的关系正在进行中

中文关键词： 乳酪乳清；抗动脉粥样硬化机制；益生菌；活性肽

英文摘要： Xinjiang Traditional fermented cheese whey (TFCW) in the Kazakh folk long-term use for regulating blood lipid, blood glucose. Our Research Group found the antiatherosclerosis effect of TFCW in experimental model. To establish its effective parts and action mechanism .First of all, the study is on isolation and purification of fermentative bacteria,then combined with morphological, physiological and biochemical characteristics and 16SrDNA, 16SrRNA, 26SrDNAD1/D2 genes identification sequence analysis method. Determine TFCW contains two valuable microorganisms: lactic acid bacteria and yeast; lactic acid bacteria including:L.brevis,L.kefianofaciens,L.helveticus, L.casei,L.plantarum,L.farciminis,L.lacti; yeast including S.unisporus, I.orientalis. Second our study is on the extraction separation of TFCW and found that the molecular weight of 1071-724Da peptide on ACE inhibition rate is of 56.45%; Molecular weight distribution of 645-500Da peptide on free radical DPPH o, and o OH, and O2-o inhibition rate is 59.06%. The third section we found that the antiatherogenic mechanism of TFCW is about regulating blood lipid, rising of LCAT activities, increasing NO content, enhancing o OH purge capabilities, as well as enhancing HDL levels and strengthening the scavenger SR-BI receptor interaction, significantly reduce CRP . In addition mechanism involved is that TFCW rich in monounsaturated fatty acid, serine, magnesium, calcium, and potassium etc, the effect has relationship with PPARs is still in progress of our research.

英文关键词： fermented cheese whey; antiatherosclerosis mechanism;Probiotics;Bioactive peptides