miRNA调控胃癌特异性腹膜转移的机制研究

项目名称： miRNA调控胃癌特异性腹膜转移的机制研究

项目编号： No.81472699

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李晓华

作者单位： 中国人民解放军第四军医大学

项目金额： 64万元

中文摘要： 由于胃癌特异性腹膜转移缺乏有效的诊疗手段，目前已成为进展期胃癌患者死亡的主要原因。因此，寻找胃癌腹膜转移的特异性分子标记物对进展期胃癌诊断和靶向治疗具有重要临床意义。我们前期研究发现miR-181b、miR-223和miR-136在特异性腹膜转移胃癌细胞系中差异表达，进而筛选并验证了其下游靶基因ADAM11、EPB41L3、ALCAM 和H0XC10。本项目拟在前期工作基础上，观察通过体内外实验观察目标miRNA调控胃癌腹膜转移恶性生物学表型的内在机制，以期找到胃癌特异性腹膜转移的逆转分子靶标；同时利用临床胃癌组织标本验证目标miRNA对胃癌腹膜转移的分子标志物作用。本项目从调控基因的上游分子miRNA的角度，探讨胃癌特异性腹膜转移的分子机制，为找到新的预警标志物及调控靶点，进一步提高胃癌腹膜转移的早期诊断率，并为消除或逆转胃癌特异性腹膜转移奠定丰富的临床和理论基础。

中文关键词： C07_胃肿瘤；miRNA；腹膜转移

英文摘要： Peritoneal dissemination is the overwhelming cause of mortality in patients with gastric cancer. The absence of specific markers for peritoneal metastasis in gastric cancer has made the successful early diagnosis and target therapy for advanced gastric cancer very difficult. Therefore, it's very important to search specific molecular markers and signal pathway for gastric cancer peritoneal metastasis. Our previous study found that miR-181b, miR-223 and miR-136 were differentially expressed in specific peritoneal metastasis of gastric cancer cell lines, and then screened and verified its downstream target gene ADAM11, EPB41L3, ALCAM and H0XC10. This project intends to observe the three miRNA how to regulate gastric cancer peritoneal metastasis, and further validation their early diagnosis and prognosis function with clinical samples. This project wants to find new targets and warning marker for gastric cancer peritoneal metastasis, and further lay a wealth of clinical and theoretical basis to eliminate or reverse specificity of peritoneal metastasis.

英文关键词： gastric cancer;miRNA;peritoneal metastasis