miRNA网络系统调控拟南芥幼年向成年转变的分子机制

项目名称： miRNA网络系统调控拟南芥幼年向成年转变的分子机制

项目编号： No.31470063

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 吴刚

作者单位： 浙江农林大学

项目金额： 30万元

中文摘要： 植物营养生长是植物发育进程中一个非常重要的环节，它调控着一系列重要的生理生化及后续的生殖发育过程。该过程由幼年营养生长和成年营养生长构成。植物如何实现幼年向成年转变决定着植物是否能正常完成营养生长这一重要的发育进程。微小RNA miR156是调控植物幼年向成年转变的主要调控因子，同时它也调控着许多其它重要的生理生化过程。然而，人们对miR156是如何受上游因子所调控却知之甚少。我们初步研究发现，先前被人们认为受赤霉素GA调控的miR159及其靶基因作用于miR156上游，与其它miRNA， 如miR319, 一起形成复杂的miRNA调控网络来调控拟南芥幼年向成年转变。本项目将采用遗传学、分子生物学、生化等方法来研究这些miRNA网络系统在调控拟南芥幼年向成年转变过程中的功能以及各个网络间的遗传与分子关系，为今后研究植物营养生长的分子机制提供理论基础。

中文关键词： miR156；miR159；miR319；营养时期转换；拟南芥

英文摘要： Vegetative development comprises an indispensable phase of development in plants by regulating a serial of physiologically and biochemically important processes as well as reproductive development. Vegetative development consits of juvenile development and adult development.How the juvenile-to-adult transition is achieved determines if the vegetative development can be successfully achieved. miR156 is the master regulator of vegetative development in plants.However, how miR156 is regulated remains largely unknown. Our preliminary work show that miR159, a miRNA previously considered to be regulated by gibberellin, as well as its target, MYB genes, acts upstream of miR156. miR159 and miR156, together with miR319, consitute a complex regulatory network to regulate juvenile-to-adult transition in Arabidopsis. How these miRNAs function and how they organize into a complex network will be studied in this project to elucidate their roles in vegetative development by using gentic, molecular, biochemical approaches.

英文关键词： miR156；miR159；miR319；vegetative phase change；Arabidopsis