REP1基因调控水稻内稃细胞分裂分化分子调控网络的研究

项目名称： REP1基因调控水稻内稃细胞分裂分化分子调控网络的研究

项目编号： No.31270222

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 袁政

作者单位： 上海交通大学

项目金额： 80万元

中文摘要： 水稻花发育不仅具有形态结构特异性，其后续形态建成还直接影响到物种的后代繁殖和产量，对其发育调控机理的研究具有重要的理论和应用价值。我们前期研究结果显示，水稻rep1突变体内稃属性部分丧失（维管束由3个变成类似外稃中的5个），后期细胞分裂分化异常（内稃变小且不能进行刚毛状细胞分化的发育）。这种变化直接影响到rep1突变体后期种子的发育和产量，种子小而畸形，该材料是进行水稻花形态建成及内稃细胞发育分子机制研究的理想材料。本项研究将以野生型和rep1突变体为研究对象，系统分析水稻外/内稃细胞发育过程；通过生物信息学和分子生物学方法筛选组织特异性细胞周期基因；通过Q-RT PCR的方法寻找受REP1特异调控的候选细胞周期基因；并通过生物化学和遗传学的方法确定REP1调控水稻内稃细胞发育的分子机制；同时进行酵母双杂交和遗传回复子筛选，为进一步揭示水稻花器官形态建成及内稃细胞发育分子调控网络奠定基础。

中文关键词： 内稃；边缘组织；REP1；OsMADS6；OsMADS32

英文摘要： Rice possess unique reproductive organ and flower morphology different from that of dicotyledon and non-grass monocotyledon. Moreover, the delicate process for the reproductive organ establishment and development not only affects its breeding directly, but also plays important role for staple food output. Therefore, researches on molecular mechanism of crop flower development will provide us both theoretical and practical knowledge for future application. In precious study, we isolated the rep1 mutants, which show palea defective growth specifically. Compared with the wild type plants, the rep1 paleas grow 5 vascular bundles instead of 3, which mimic growth pattern in lemma. Furthermore, the palea cells are smaller and lack trichomes differentiation in the rep1 mutant. These abnormal growth directly affect palea development and seeds production in the rep1 mutant. In this study, to further characterizing the REP1 gene function in controlling palea development, palea cell proliferation and differentiation process is firstly been observed in series developmental stage by SEM and paraffin sections. Secondly, core cell cycle genes are selected through bioinformatics analysis, and their expression pattern was analyzed by RT and in-situ hybridization. Finally, these putative target genes of REP1 was analyzed through m

英文关键词： Palea；margin tissue of palea；REP1；OsMADS6；OsMADS32