板栗壳原花青素诱导肿瘤细胞自噬性死亡及其机制研究

项目名称： 板栗壳原花青素诱导肿瘤细胞自噬性死亡及其机制研究

项目编号： No.31201456

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 膳食与营养

项目作者： 段玉清

作者单位： 江苏大学

项目金额： 26万元

中文摘要： 从植物中寻找高效低毒抗肿瘤作用的活性成分一直是世界抗肿瘤药物研究热点。活性成分诱导肿瘤自噬性死亡的研究是目前肿瘤治疗的新策略，业已证实原花青素是能促进多种肿瘤细胞凋亡的活性成分，且安全无毒，但其诱导肿瘤自噬性死亡活性及机制研究未见报道。基于此，本项目拟以板栗壳中原花青素(CSPCs)为对象，针对化学组成、剂量效应和时间效应对其诱导肿瘤细胞自噬性死亡活性不清楚，作用机制缺乏研究的现状，拟在确定CSPCs组成和含量后，以人肝癌HepG2为细胞模型，研究CSPCs诱导人肝癌细胞自噬性死亡作用。重点研究CSPCs对P?3K/Akt/mTOR通路的3个主要效应分子的表达、Beclin 1和与之相关的Bcl-2家族成员及其对LC3П表达量的影响，揭示CSPCs诱导肿瘤细胞自噬性死亡活性和分子机制，为深化我国特色植物资源和农产品加工副产物，开发高活性植物成分提高国民健康提供理论依据。

中文关键词： 板栗壳；原花青素；自噬；凋亡；分子机制

英文摘要： It is always a hotspot for tumor treatment research to look for efficiency and low toxicity antitumor drugs from plants. Autophagic death in tumor cells induced by active ingredient may provide novel strategies for the treatment of cancer treatment. Studies have proved that procyanidins is safe and nontoxic, which can promote the apoptosis of tumor cells. But the autophagic death in tumor cells induced by procyanidins and its mechanism remain unclear. Based on this fact, in view of the chemical composition, the mechanism, and the dose and time-dependent effect of procyanidins from chestnut shell (CSPCs) on the activity of autophagic death need further research. The composition and content of CSPCs will be determined, and then the autophagic death of human hepatoma HepG2 cell induced by CSPCs will be investigated in the project. This study mainly researches the effect of CSPCs on the express of three major effector molecule in phosphatidyl inositol 3-kinase/protein kinase B/mammalian target of Rapanycin, (P?3K/Akt/mTOR) pathway, Beclin 1 and associated Bcl-2 family members to the expression level of microtubule-associated protein 1 light chain 3 П (LC3П), attempts to reveal the molecular mechanism of tumor cell autophagic death induced by CSPCs, and provides a theoretical basis for the development of highly activ

英文关键词： chestnut shell；procyanidins；autophagy；apoptosis；molecular mechanism