基于空间分辨的芯片电泳高效检测方法研究

项目名称： 基于空间分辨的芯片电泳高效检测方法研究

项目编号： No.21305089

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 倪一

作者单位： 华东理工大学

项目金额： 25万元

中文摘要： 芯片电泳是实现高效基因分析的重要技术之一。芯片电泳的高效率来自于系统"尺度"的减小，其有效长度和进样宽度比毛细管电泳缩短一至两个数量级。然而，现有荧光检测方法的"尺度"与芯片电泳并不匹配，限制了芯片电泳效率的进一步提高。为此，本项目拟提出一种新型诱导荧光检测方法，可以实现亚微米量级的检测宽度，比现有技术降低二至三个数量级。该方法以电荷耦合器件（CCD）的空间分辨能力为基础，利用CCD单个像素的小尺寸实现极小的检测宽度，利用像素间的关联性提高检测灵敏度。项目将建立DNA电泳检测模型，计算荧光信号的空间分布与变化，分析像素间的关联性，提出信号处理算法。通过模型，系统地研究新方法的条件参数，分析并归纳各项参数的影响规律。构建实验系统，进行算法测试，对方法特性进行实验测量。针对实验室高分辨应用与现场便携式应用研制两套CCD芯片电泳检测系统。研究成果将为进一步提高芯片电泳效率提供新的途径。

中文关键词： 芯片电泳；电荷耦合器件；检测宽度；；

英文摘要： Chip electrophoresis is one of the most promising candidates of high efficient genetic analysis. The origin of the high speed and high efficiency of chip electrophoresis is "scaling factor". Both effective length and sample width of chip electrophoresis are one or two orders of magnitude smaller than those of capillary electrophoresis. However, detection width describing the efficiency of fluorescence detection system is not correspondingly improved, which hinders the improvement of the efficiency of chip electrophoresis. In order to take full advantage of chip electrophoresis, a new fluorescence detection technique is developed. On the basis of charge coupled device (CCD), ultra-small detection width and improved signal-to-noise ratio are achieved due to the small size of single pixel of CCD and the pixel array of CCD, respectively. Submicron detection width, which is two to three orders of magnitude smaller than that of the conventional detection methods, is achieved by using the new detection technique. Model of DNA electrophoresis detection is built to analyze the relation among signals from different pixels. Systematically investigation of the properties of the new detection technique is carried out, and the influences of the parameters are explored. Experiment system is constructed. Two CCD based chip elec

英文关键词： chip electrophoresis；charge-coupled-device；detection width；；