天然三倍体鲫鱼萍乡红鲫精卵识别相关因子SP56-like与ZP3功能互作研究

项目名称： 天然三倍体鲫鱼萍乡红鲫精卵识别相关因子SP56-like与ZP3功能互作研究

项目编号： No.31260282

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 王军花

作者单位： 南昌大学

项目金额： 50万元

中文摘要： 雌核发育是卵子在精子的刺激下，依靠自身的细胞核发育成个体的一种有性生殖方式，精子仅起到刺激卵核发育并不参与卵核融合。萍乡红鲫是江西萍乡地区发现的一种天然的野生型三倍体鲫鱼突变体，是行雌核发育和两性生殖的两性型种群，且种群内暂未发现异精效应，与银鲫不尽相同，暗示着该鲫鱼突变体具有较复杂的生殖调控机制。为进一步了解其生殖遗传机制，本研究结合文献报道在哺乳动物中SP56作为卵透明带蛋白ZP3的受体参与精卵识别的基础上，将从萍乡红鲫中克隆其ZP3的受体SP56-like基因，分析其时空表达特征；并结合已有的工作基础（已克隆到ZP3基因并获得其抗体），采用免疫共沉淀技术和竞争受体试验从体外和体内两方面研究萍乡红鲫SP56-like是否与ZP3相互作用，以及它们与受精识别和雌核发育的关系。其研究对于探索和揭示萍乡红鲫受精生殖分子调控机制具有重要意义。

中文关键词： 萍乡红鲫；ZP2；ZP3；Hira；SP56

英文摘要： A form reproduction in which the egg can inhibit heterologous sperm from transforming into male pronucleus so that ontogenesis is completely controlled by the female nucleus is thought as gynogenesis. A red-transparent population, distributed in Pingxiang of Jiangxi Province(termed as Pingxiang Red crucian carp), was identified to be natural triploid Carassius auratus by our group.The species exists a bisexual population, female outnumbering male by about six to one in their natural habits,and was also demonstrated that it has two different reproductive development modes including bisexual reproduction involving the fusion of male and female gametes and gynogenesis reproduction by means of artificial propagation experiments in our previous study. In comparision with naturally gynogenetic silver crucian carp, no evidences have not yet for the present been found the obvious allogynogenetic biological effects in growth, rate of fertilization and other aspects exist in Pingxiang Red crucian carp, which suggested that this red-transparent triploid Carassius auratus possesses more complicated molecular mechanism and regulative pathway in the reproduction and development. To better understanding the special genetic mechanism in Pingxiang Red crucian carp, in this study, the homologous genes to sperm protein 56(SP56-l

英文关键词： Carassius auratus var. Pingxiangnensis；ZP2；ZP3；Hira；SP56