BAS-SPR多级增强对典型BMZs杀菌剂的高效识别机理

项目名称： BAS-SPR多级增强对典型BMZs杀菌剂的高效识别机理

项目编号： No.31301487

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 宋洋

作者单位： 天津师范大学

项目金额： 23万元

中文摘要： 苯并咪唑类(BMZs)杀菌剂具有高效、低毒、广谱的特点。由于在实验动物和靶动物中显示的致畸和致突变作用，BMZs成为了食品残留中重要的监控对象，更加严格的限量标准也将相继颁布。SPR技术因样品前处理简单，人为干扰因素少，自动化程度高等优点，特别适合大量样品的食品检测。然而SPR对小分子识别信号不高、检测灵敏度和准确度相对较低，以及过分依赖抗体质量，阻碍了其在超灵敏检测中的应用。因此，如何提高SPR对小分子的识别信号，从而提高检测的效率及准确性，对其在食品安全检测中的应用具有重要的意义。本项目在传感器芯片表面原位合成BAS信号放大敏感膜作为识别元件，以提高SPR对小分子的识别信号，从而构建BAS信号放大SPR传感器。另外，本项目采用Langmuir动力学吸附模型，结合反应物之间的图谱信息，从分子层面进一步揭示BAS敏感膜的高效识别机理，为信号放大策略在SPR传感器中的运用提供一定的理论支持。

中文关键词： 杀菌剂；表面等离子共振传感器；生物素-亲和素；分子识别；信号放大

英文摘要： Benzimidazole (BMZs) fungicides with the characteristics of high efficiency, low toxicity, and broad-spectrum, becomes a key food residue monitoring object. Since the teratogenic and mutagenic effects were found in laboratory animals and target animals,the more stringent limits will also be promulgated. SPR technology was especially fitting for the large numbers of sample analysis due to the advantage of simple pre-treatment process and less human disturbance with high degree of automation. However, the bottlenecks of weak response signal and low accuracy on small molecules, as well as over-reliance on antibody quality, has impeded the application in ultra-sensitive detection for the surface plasmon resonance sensor (SPR) technology. How to improve the SPR signal identification of small molecule target to enhance the efficiency and accuracy of detection is taking an important role in food safety testing. The study is designed using the sensitive membrane of the sensor chip surface in situ synthesis of biotin-avidin system (BAS) as a recognition element with the identification signal of small molecule to improve the SPR signal identification of small molecules thus to develop the BAS signal amplification SPR sensor. In addition, the Langmuir kinetic adsorption model, with the reactants map information between th

英文关键词： fungicide；Surface plasmon resonance；Biotin avidin；Molecular recognition；Signal amplification