基于生物打印技术研制生物活性可降解牙周缺损修复材料

项目名称： 基于生物打印技术研制生物活性可降解牙周缺损修复材料

项目编号： No.51502238

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 一般工业技术

项目作者： 马玉菲

作者单位： 西安交通大学

项目金额： 20万元

中文摘要： 我国80%以上成年人患有不同程度的牙周病，它会引发牙周组织缺损，破坏牙槽骨。因此，修复牙周缺损是治疗牙周病亟待解决的问题，具有重要的学术价值和社会意义。目前，国内外针对牙周缺损修复材料（PDRM）的设计均源于传统的骨修复，无法完全满足缺损修复的临床需求。项目拟用生物打印技术，基于PDRM诱导干细胞定向分化的评价，研制专用于牙周缺损修复的生物材料。首先，拟采用两种具有光交联特性的材料制备水凝胶并通过表征其生物相容性及降解性，筛选最佳材料组成。其次，利用生物打印平台研究基于珍珠水溶性有机质、原位矿化磷酸钙盐及光交联水凝胶构成的几种PDRM对人牙龈间充质干细胞成骨分化的作用，探讨材料特性对干细胞分化的影响。最后，建立犬牙周病模型并评价PDRM的体内修复能力。该项目将组织工程与生物打印技术相结合，探讨一种全新的细胞/材料相互作用的研究方法，并研制新型PDRM，以期为牙周缺损修复的临床应用奠定基础。

中文关键词： 支架；间充质干细胞；生物活性因子；细胞成骨分化；生物功能

英文摘要： More than 80% adults in China suffer from the periodontal disease with varying degrees. Periodontal disease can result in periodontal defects, which leads to alveolar bone destruction. Hence, repair of periodontal defects is an urgent problem to be deal with in the field of periodontal disease treatment, which has an important academic value and social significance. At present, the design of periodontal defects repair materials (PDRM) is originated from the traditional method of bone repair, which is unable to fully meet the clinical requirements for periodontal defects repair. In this project, bio-printing technology and method of tissue engineering were employed to develop a kind of biomedical material dedicated to repairing periodontal defects based on the evaluation of stem cells differentiation. First of all, two kinds of the photo-crosslinking macromolecules were used to prepare hydrogel, and then the optimal material composition of the hydrogel was selected by charactering the biocompatibility and degradability. Secondly, the effect of several PDRM based on pearl water soluble matrix, in-vitro mineralized calcium phosphate and the photo-crosslinking hydrogel on human gingival mesenchymal stem cells differentiation to osteoblasts was investigated by the bio-printing platform. At last, dog periodontal disease model was built to evaluate the in-vivo repair ability of PDRM. Tissue engineering and bio-printing technology were combined in this project to discuss a novel method to investigate the cell/material interaction, develop a new material for repairing periodontal defects and lay the foundation for its clinical application.

英文关键词： scaffold;mesenchymal stem cells;bioactive factor;osteogenic differentiation;biofunctional