海岛棉水杨酸途径脂肪酶基因GbEDS1抗黄萎病功能及分子机制

项目名称： 海岛棉水杨酸途径脂肪酶基因GbEDS1抗黄萎病功能及分子机制

项目编号： No.31301371

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 张艳

作者单位： 河北农业大学

项目金额： 23万元

中文摘要： 抗病育种是防治棉花重要病害-黄萎病的经济有效措施，研究抗黄萎病分子机制对棉花抗病育种具有重要意义。项目组从黄萎病菌诱导的海岛棉全长cDNA文库分离了Enhanced Disease Susceptibility 1 （EDS1）基因，初步研究表明转基因拟南芥对黄萎病具有很好的抗性。本研究拟对GbEDS1进行抗黄萎病作用机制解析。研究棉花分别受黄萎病菌和信号分子处理后基因的表达；构建VIGS表达载体并转化棉花，反向快速验证基因功能，并进一步研究该基因对不同抗病途径重要基因（水杨酸：PR1a、PR5a；茉莉酸：PR4；乙烯：EREBP； ROS：CAT1、GST、APX）的影响；构建真核过表达载体转化感病陆地棉，研究转基因材料黄萎病菌胁迫下基因表达和抗性反应；分析GbEDS1基因超表达对抗病内源性激发因子乙烯、水杨酸和H2O2表达量的影响，探明基因的抗病作用及机制，为抗黄萎病育种提供理论依据。

中文关键词： 棉花；黄萎病；GbEDS1基因；功能；抗病机制

英文摘要： Breeding resistant variety is an efficient strategy to control cotton disease.It is important for cotton breeding community to ascertain the molecular mechanism against Verticillium wilt. A gene homologous to EDS1 (Enhanced Disease Susceptibility1) was isolated from full-length cDNA library of G. barbadense induced by V.dahliae. The marked Verticillium wilt resistance was observed in transgenic Arabidopsis. In this study, the molecular mechanism analysis of GbEDS1 will be carried out. The cotton expression pattern of the EDS1 will be conducted by using Real-time PCR when induced by V. dahliae pathogen and signal molecules, respectively. Virus-induced gene silencing (VIGS) expression vector will be employed to transform cotton and silence endogenous EDS1 gene of G. barbadense, and to mine cotton metabolic pathways that the EDS1 participates in by detecting expression of some marker genes, such as PR1a and PR5a in SA, PR4 in MeJA, EREBP in ET and CAT1, GST, APX in ROS pathways. Furthermore, we will construct GbEDS1-pBI121 overexpressing vector and transform susceptible upland cotton for analyzing the resistance of transgenic plants under V. dahliae stress and investigate the effects of GbEDS1 overexpression to ethylene(ET), salicylic acid(SA) and hydrogen peroxide(H2O2). These will be of significance to understand

英文关键词： Cotton；Verticillium wilt；GbEDS1 gene；function；resistance mechanism