含蒽大环多胺的合成及其对核苷酸的识别和传感研究

项目名称： 含蒽大环多胺的合成及其对核苷酸的识别和传感研究

项目编号： No.21472066

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 环境科学、安全科学

项目作者： 冯国强

作者单位： 华中师范大学

项目金额： 85万元

中文摘要： 由于核苷酸的生物重要性，发展它们的受体分子以及对它们进行分子识别和传感具有重要的研究意义。然而，核苷酸类磷酸酯品种多样而又结构相似，同时在水相中又容易高度溶剂化，使得它们在水相中的高选择性和高灵敏度识别成为难题。本项目试图利用蒽环的荧光性能，通过结合强的金属配位作用和蒽环与核苷酸上某些碱基的π-π相互作用，同时结合大环空腔的大小调节这种新模式，来发展结构新颖的含蒽大环多胺受体分子，并实现它们对核苷酸高选择性、高灵敏度的识别和传感。为此，本项目拟制备出结构多样并具有不同空腔大小的含蒽大环多胺化合物，系统地研究它们对阴离子的识别，并通过大环结构的不断优化，找到能在水相体系中高效识别核苷酸的主体化合物，同时希望能在分子水平上揭示含蒽大环多胺与核苷酸等阴离子的识别作用规律，为解决核苷酸识别中选择性难等问题提供新的思路与方法，也为以后进一步的磷酸酯识别和水解研究打下基础。

中文关键词： 蒽；大环多胺；核苷酸；阴离子识别；化学传感

英文摘要： The development of receptors for nucleotides and study their molecular recognition are highly important due to their biological significance. However, the strong hydration of these anions in water and the structural similarity of the phosphate anion family, make the creation of highly selective and sensitive receptors for nucleotides in aqueous solution highly challenging. In this proposal,anthracene-containing macrocylic polyamines as new receptors for recognition and sensing of necleotides are proposed to be developed,and a new approach of combining the strong metal coordination interactions with the defined cavity size of macrocylic polyamine,and with the π-π stacking interactions between the fluorescent anthracene moiety and the nucleic base is proposed to achieve high selectivity and sensitivity.To realize this, we will synthesize a series of structurally diversified anthracene-containing macrocylic polyamine receptors with different cavity size and different functinal groups, and will systematically study these new receptors for recognition and sensing of necleotides.Through continuous rational modification of the receptor's structure, this project is expected to find out some effective receptors for nucleotides, and to uncover the possible rules of the selectivity between the cavity size of macrocylic receptors and the structure of anions of interest. Besides,this project is also expected to provide new insight on how to create effective receptors for nucleotides in aqueous solution, and to pave the way for the further related study on phosphate recognition and hydrolysis.

英文关键词： anthracene;macrocyclic polyamine;necleotide;anion recognition;chemosensing