分子光开关用于嵌段共聚物自组装纳米结构的超分辨荧光成像

项目名称： 分子光开关用于嵌段共聚物自组装纳米结构的超分辨荧光成像

项目编号： No.21474034

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 朱明强

作者单位： 华中科技大学

项目金额： 87万元

中文摘要： 嵌段共聚物自组装纳米结构，其形貌和尺寸通常采用电镜或原子力显微镜进行 表征。近年来人们发展了一种基于单分子光开关荧光探针高准确定位的超分辨荧光显微技 术，可突破光学衍射极限，分辨率达到纳米尺度（最低至几个纳米）。本研究基于随机光学 图像重构（Stochastic optical reconstruction microscopy，STORM）或光活化定位显 微术（Photo-activated localization microscopy, PALM）的基本原理，发展新兴荧光分 子开关，对这些新型荧光分子开关进行分子相容性及光学开关优化，建立起一套适用于超分 辨显微成像的荧光开关分子探针库，研究可用于不同嵌段共聚物的特异性超分辨成像探针，并对不同嵌段共聚物自组装纳米结构进行超分辨光学成像，将光学成像分辨率提高至20 纳米尺度，实现嵌段共聚物的纳米级光学分辨表征。

中文关键词： 荧光分子开关；超分辨成像；嵌段共聚物；螺吡喃；自组装

英文摘要： Self-assembly of block copolymers produces microphase separation and periodic nanostructures, which are generally characterized by TEM, SEM or AFM. Recently, a super-resolution fluorescent imaging method was developed based on high-accurate localization of single photoswitchable fluorophore, which can surmount the optical diffraction limit affording nanoscale optical resolution down to several nanometers. Based on the mechanism of stochastic optical reconstruction microscopy (STORM) or photo-activated localization microscopy (PALM), this project is to develop novel fluorescent molecular switches with optimal optical switching and compatibility to block copolymers, construct a library of fluorescent molecular switches for super-resolution imaging, optimize the specific super-resolution imaging probes for various block copolymers and conduct the super-resolution imaging experiments of self-assembled nanostructures of block copolymers to improve the optical imaging resolution. Inspired by the success application of super-resolution imaging in subcellular nanostructures, we hope that the multidisciplinary integration of chemistry and optical science would provide 20 nm of imaging resolution for self-assembled nanostructures of block copolymers.

英文关键词： fluorescent molecular switches;super-resolution imaging;block copolymers;spiropyran;self-assembly