多功能钌配合物磷光探针的研制及其在细胞器生理活性组分靶向定位识别中的应用

项目名称： 多功能钌配合物磷光探针的研制及其在细胞器生理活性组分靶向定位识别中的应用

项目编号： No.21475015

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 张文珠

作者单位： 大连理工大学

项目金额： 85万元

中文摘要： 钌配合物磷光探针用于活细胞成像测定具有荧光寿命长、稳定性好、Stokes位移大、可用可见光激发等优点，而开发功能更强大、适用范围更广的新型探针一直是钌配合物磷光探针重要的研究内容。本研究以研制多功能的钌配合物磷光探针为研究目标，拟通过合理的结构设计，合成一系列可靶向定位识别细胞器中生理活性分子或离子、及可同时检测几种生理活性分子或离子的多功能钌配合物磷光探针。结合荧光显微镜成像技术，建立活细胞内细胞器中生理活性分子或离子的时间分辨磷光显微成像测定新方法，及使用一种探针同时定量测定活细胞内几种生理活性分子或离子的检测平台。本项目的开展预期将开拓钌配合物磷光探针新的研究与应用领域，预期构建的分析方法与技术将对研究细胞器的功能、了解细胞内生理活性组分与疾病的关系、荧光影像学进步、新药开发以及分析化学的学科建设起到积极的推动作用。

中文关键词： 多功能钌配合物磷光探针；生物活性分子及离子；细胞器；靶向定位识别；时间分辨磷光成像

英文摘要： There are several advantages for Ru(II) complex-based phosphorescent probes in cell imaging detection, such as long lifetime, good stability, long Stokes shift, visible-light excitation, and an important research field for the Ru(II) complex-based phosphorescent probes is the development of multifunctional and more applicable probes. The aim of this project is to develop various multifunctional Ru(II) complex-based phosphorescent probes. By rational design of molecular structure, a series of the multifunctional Ru(II) complex-based phosphorescent probes will be synthesized for the location recognition bio-active molecules and ions in organelles, and the simultaneous detection of several targeting molecules, respectively. Combined with microscopy imaging technique, time-gated phosphorescence imaging assays for bio-active molecules and ions in organelles will be established. The research of this project is expected to extent the new research and application fields of Ru(II) complex-based phosphorescent probes. The established assay techniques will also play an active role for promoting the researches on functions of organelles and relationship of bio-active species with disease in living cells, and progess of fluorescence imaging, as well as the development of analytical chemistry.

英文关键词： Multifunctional ruthenium(II) complex-based phosphorescent probe;bio-active molecules and ions;organelles;target-location recognition;time-gated phosphorescence imaging assay