抗炔草酯的棒头草种群开花提前调控机制研究

项目名称： 抗炔草酯的棒头草种群开花提前调控机制研究

项目编号： No.31501658

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 农业科学

项目作者： 周凤艳

作者单位： 安徽省农业科学院

项目金额： 20万元

中文摘要： 揭示杂草抗性进化机制对于杂草防治的应用意义重大，但抗性杂草生殖生长阶段的进化机制尚不明确，而开花时间的严格调控对植物繁殖成功至关重要，可增强种子发育的竞争力。项目申请人发现棒头草抗性种群QS与敏感种群XC开花时间存在差异，以此为基础，推测可能是由于QS和XC开花调控基因存在差异，导致该基因以某种形式调控了植物体内抽穗、结实相关基因的表达，进而影响了抗性种群花序和结实量的改变，进化并增强了其在环境中的竞争力。本项目拟通过Illumina测序和生物信息学分析，构建棒头草QS和XC种群开花初期mRNA转录组比对信息，分析获得差异表达开花调控基因；qRT-PCR法验证表达量差异，进而筛选与抗性相关的开花调控基因；并运用拟南芥转基因技术和酵母双杂交系统，分别从活体和细胞水平研究目标基因在棒头草抗性进化机制中的作用。项目的开展将为除草剂抗性进化机理研究提供新的探索途径和理论依据。

中文关键词： 抗药性杂草；抗药性；生长发育；繁殖

英文摘要： It is of great significance to understand the resistant mechanism in weeds to enable their control; however, the evolution mechanism in weeds during the reproductive stage is not clear yet. The strict regulation of flowering time is crucial for plant breeding, which can enhance competitiveness for seed development. The applicant has found that Polypogon fugax resistant population (QS) and sensitive population (XC) have different flowering times, and the phenomena may be caused by the differences in flowering time genes. Subsequently, the expression of the plants’ heading- and firming-related genes are regulated by the flowering time gene in some way, thus resulting in changes in the amount of inflorescences and seeds in the resistant population, which influences its evolution and enhances its competitiveness in the environment. This project aims to construct mRNA profiles from the early flowering stage in QS and XC populations by Illumina sequencing and bioinformatics analysis, and then to obtain the differentially expressed flowering genes by comparative analysis. The results will be validated using qRT-PCR, and the flowering genes that are related to P. fugax resistance will be screened. Finally, the role of these identified genes will be explored both in vivo and in vitro using transgenic Arabidopsis and the yeast two-hybrid system. This project will provide new information and a theoretical basis for the evolutionary mechanism to herbicide resistance.

英文关键词： herbicide resistant weed;herbicide resistance;growth ;reproduction