TWIST在胃癌多药耐药中的作用及分子机制

项目名称： TWIST在胃癌多药耐药中的作用及分子机制

项目编号： No.81201924

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 肿瘤学2

项目作者： 郭雪艳

作者单位： 西安交通大学

项目金额： 23万元

中文摘要： TWIST是bHLH（basic helix-loop-helix）转录因子超家族的成员，近年来研究示其参与多种肿瘤发生发展、转移等恶性生物学表现,并发现TWIST可能与肿瘤耐药有关，但与胃癌耐药的关系及机制不详。胃癌多药耐药（multidrug resistance, MDR）是胃癌化疗失败的主要原因之一。但目前仍未发现行之有效的逆转方法。我们前期研究表明TWIST参与胃癌发生发展，在胃癌耐药细胞SGC7901/VCR细胞中高表达。综上提示，TWIST可能参与胃癌多药耐药的形成。因此，本研究拟利用分子生物学和免疫学、TAP、蛋白质二维电泳及基因芯片等技术观察TWIST对胃癌多药耐药性的作用及机制，为深刻理解耐药肿瘤细胞抵抗化疗分子机制奠定一定的理论基础。从而为逆转耐药提供一种新方法。

中文关键词： TWIST；胃癌；多药耐药；；

英文摘要： TWIST is one of the bHLH tanslating infactor family.Recent studes show that TWIST may contribut to various properties of tumnor cells ,for example induction of tumorigenesis, promotion of invasion and metasis.TWIST was also discovered play a role in multi-drug resistance of tumor cells.To date, however, the data on the role of TWIST in the MDR of gastric cancer are still unclear.The multidrug resistanc of gastric cancer is the one of the main cause for failed chemiotherapy.But there is no useful method toreverse it. In the Our earlier study indicated the up-regulation of TWIST protein was observed in gastric cancer drug-resistant cell line SGC7901/VCR compared with GES-1 and other gasteric cancer cell lines by Western blotting and RT-PCR.The all data reveal TWIST may play a crical role in the MDR of gastric cancer.So,in this study we want to investigat the role and molecular mechanism in multidrug resistance of gastric cancer by MTT,TAP,DNA chip and so on.More related theroy will be revealed to us to have a more profound understanding in MDR of gastric cancer.This successful operation provides evidence to validate that twist-siRNA may become a new medical technique to reverse drug resistance.

英文关键词： TWIST；gastric cancer；MDR(multi-drug resistant)；；