河蟹flotillin样蛋白在CHH神经多肽激素调控血糖代谢中的作用研究

项目名称： 河蟹flotillin样蛋白在CHH神经多肽激素调控血糖代谢中的作用研究

项目编号： No.31302168

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 李冉

作者单位： 天津师范大学

项目金额： 25万元

中文摘要： 甲壳动物眼柄CHH家族神经多肽激素包括高血糖激素（CHH）、蜕皮抑制激素（MIH）、性腺发育抑制激素（GIH）等，调控着血糖代谢、蜕皮生长和性腺发育等重要的生理功能。CHH家族神经多肽激素迄今仅在甲壳动物中被发现，且尚不清楚它们的作用靶标分子、调控通路和机制。为了筛选CHH作用的靶标分子，本实验室以重要经济甲壳动物河蟹为研究对象，以切除眼柄前后的河蟹肝胰腺为样品，进行了转录组学和表达谱的初步分析，发现flotillin 样膜蛋白在糖代谢通路中发挥信号接收和传递功能，很可能起到了靶标分子的作用。本研究拟利用组学分析进一步验证CHH与flotillin样蛋白的相互关系，利用基因沉默等技术研究flotillin样蛋白对糖代谢中胞内下游信号通路的影响。预期结果有望阐明河蟹眼柄CHH调控糖代谢的关键靶标分子，为研究甲壳动物眼柄神经多肽激素调控糖代谢的复杂网络和人工控制河蟹的代谢生长提供参考。

中文关键词： 甲壳动物升血糖激素；甲壳动物升血糖激素结合蛋白；糖代谢；激素受体；细胞信号

英文摘要： Crustacean hyperglycemic hormone (CHH) and its structurally related peptides, molt-inhibiting hormone (MIH), gonad-inhibiting hormone (GIH), form a neuropeptide family in crustaceans called the CHH-family. Members in this family play a pivotal role in the regulation of many important biological events including carbohydrate metabolism, molting, and gonadal development. To date, the CHH-family members have only been discovered from crustaceans. Besides, the targets of these hormones and their function have not been clearly identified. Transcriptomics analysis of genes expression followed by artificial eyestalk ablation of the mitten crab was carried out by our team. Among many unigenes which show great changes in the expression level, we select one gene which expressed membrane protein closely related to CHH, flotillin. In vertebrate, this protein usually acts as a signal station which transfers the signal from the outside of the cell into the inside. This project will choose the Chinese mitten crab, which is an important economic crustacean, as our research object and parse the molecular structure of this flotillin-like protein. With further transcriptomics analysis, we will try to verify the the binding activity between CHH and flotillin. Besides, the impact on the downstream signaling pathway of flotillin will

英文关键词： CHH；CHHBP；glucose metabolism；hormone receptor；cell signaling