拟南芥染色质重塑因子AtBRAHMA维持主根干细胞微环境的机理研究

项目名称： 拟南芥染色质重塑因子AtBRAHMA维持主根干细胞微环境的机理研究

项目编号： No.31201106

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 发育生物学与生殖生物学

项目作者： 杨松光

作者单位： 中国科学院华南植物园

项目金额： 23万元

中文摘要： 染色质重塑复合体通过其具有ATPases活性的亚基水解ATP释放能量，改变核小体构象而改变DNA的"可及性"，进而调控基因表达。AtBRAHMA（AtBRM，推测的染色质重塑复合体亚基），参与植物开花、花器官发育和种子成熟等多种生理过程，但至今未见AtBRM调控主根发育的报道。我们发现AtBRM缺失突变体brm3主根变短，QC（quiescent centre）干细胞活性降低，初步表明AtBRM通过维持主根干细胞微环境调控主根发育。本项目拟在此基础上，将维持主根干细胞微环境报告基因导入brm3中，确定brm3中相关基因表达量及位置变化；通过转录因子文库和染色质因子的酵母双杂交筛选，确定与AtBRM发生互作的蛋白；利用ChIP-seq技术，确定AtBRM的靶基因。以期阐明AtBRM维持主根干细胞微环境过程的分子机制，为全面解析植物染色质重塑复合体调控植物生长发育的机制奠定基础。

中文关键词： 染色质重塑复合体；根冠干细胞微环境；维持；拟南芥；

英文摘要： The large multi-subunit of chromatin remodeling complexes regulates DNA accessibility for activation or repression of transcription by using its ATP hydrolysis to modulate nucleosome state. It has been reported that AtBRM,the putative subunit of chromatin remodeling complexes plays a central role in regulation of flowering time control, floral organ development and seed maturation. Nevertheless,less is known about AtBRM in the regulation of root development.Our existing data showed that brm3,the AtBRM loss-of-function mutant, displayed a shorter root phenotype and reduced QC activity, which suggested the involvement of AtBRM in root stem cell niche maintenance in Arabidopsis. In this study, we concentrated on the mechanism characterization of root stem cell niche maintenance by AtBRM, and a series resarch as the follows will be involved.1. To investigate the expression and localization of genes related to root stem cell niche maintenance in brm3 mutant.2.To investigate the AtBRM-interacting transcriptional factor (chromatin factor) through yeast two hybridization screening; 3. To investigate the direct AtBRM-interacting genes in Arabidopsis by means of ChIP-seq, especially those gene involved in root stem cell niche maintenance.Based on the research above, we try to clarify the molecular mechanism of AtBRM in ro

英文关键词： chromatin remodeling complex；root stem cell niche；maintenance；Arabidopsis；