基于连续切片的兔心传导系统数字三维重建及起搏通道立体定位

项目名称： 基于连续切片的兔心传导系统数字三维重建及起搏通道立体定位

项目编号： No.81200068

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学一处

项目作者： 温昱

作者单位： 中国医科大学

项目金额： 23万元

中文摘要： 心传导系统结构、功能改变引起的心律失常是常见病，轻者降低患者生活质量，重者导致心源性猝死。随着心律失常治疗工作的广泛开展，心传导系统结构改变与心电生理特性的关系逐渐引起了人们的关注，阐明其三维解剖关系及微细结构，对探讨心律失常的发病机制及其防治意义重大。本课题选用不同胚龄新西兰兔整心进行连续半薄切片、心传导系统细胞特定抗原NF-160免疫组化染色后光镜检测阳性部位，及连续冰冻切片、起搏通道HCN4免疫荧光染色后激光共聚焦显微镜检测阳性表达细胞，分别获取数字化显微图像；再应用RandAlign图像配准软件及AutoQuant三维成像系统，对图像进行追踪、叠加，得到心传导系统发育过程三维立体的微细结构影像图库,阐明其微细结构形态演变规律；再比对两套图片阳性区域，探讨起搏通道在心传导系统中的立体定位情况及与周围组织排列关系。为探讨心律失常发病机制奠定实验基础，为防治心律失常提供新思路和理论依据。

中文关键词： 起搏细胞；NF-160；HCN4；HCN2；Cx43

英文摘要： The arrhythmia will dicrease the life quality, even cause cardiac sudden death,that is one of the most common disease.The disorder of the structure and function of cardiac conduction system, that is the most common reasons to cause the arrhythmia. The relationship between the structure changes and disorder of cardiac electricity physiology cause more and more attention, following the development of arrhythmia treatment work.It is meaningful significant to clarify the three dimensional anatomy and the microstructure of cardiac conduction system. We choose the New Zealand rabbit whole hearts in different embryo age as the samples, cutting it for the preparation of series semithin sections, and using NF-160 labelled. We detect the positive part within the semithin sections under light microscope. At the same time, we prepare the series frozon sections of the whole heart, using HCN4 labelled, and detect the positive cells under the Laser Confocal microscope. Then we will get two series digital imagings about NF-160 positive part and HCN4 positive cells.The next, we use the RandAlign soft and AutoQuant three dimensional imaging system to track and pile up those digital imagings, and can get the three dimensional microimagings bank of cardiac conduction system.Then compare the positive part of the two sets image

英文关键词： pacemaker cells；NF-160；HCN4；HCN2；Cx43