甘肃鼢鼠PTSD后睡眠障碍的海马神经编码机制与四逆散的干预研究

项目名称： 甘肃鼢鼠PTSD后睡眠障碍的海马神经编码机制与四逆散的干预研究

项目编号： No.81460611

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李越峰

作者单位： 甘肃中医药大学

项目金额： 47万元

中文摘要： 睡眠障碍是创伤后应激障碍（PTSD）标志性症状之一，与PTSD的发生、发展和转归密切相关。舟曲泥石流、岷县、玉树和汶川等灾区高达70～91％的PTSD患者存在严重睡眠障碍。本项目围绕PTSD睡眠障碍与强烈创伤引起大脑功能的应激损伤，海马神经元时空特性和编码机制异常，导致睡眠调节功能障碍的研究假说，以具有典型昼夜节律的黄土高原特有物种甘肃鼢鼠复制PTSD动物模型，检测分析鼢鼠异常睡眠脑电时相和特征；并基于海马神经编码理论和模式，利用在体多通道微电极阵列神经信号（M-NEMEA）技术检测PTSD睡眠障碍的海马（CA1、CA3区）神经元时空模式和编码特征改变；结合调肝（肝主疏泄）以安神（调畅情志）的中医理论和以往工作基础，进一步探讨和揭示调肝方药四逆散对PTSD睡眠障碍的海马神经元时空特性、信息编码整合处理和功能重构的干预机制，从而为中医药防治PTSD及睡眠障碍的机制研究和临床应用提供依据。

中文关键词： 甘肃鼢鼠；创伤后应激障碍；睡眠障碍；四逆散；神经编码机制

英文摘要： Sleep disorder is one of the hallmark symptoms of post-traumatic stress disorder (PTSD), it is closely correlated with the occurrence and prognosis of PTSD. PTSD patients with severe sleep disorders are as high as 70 to 91% in Zhouqu mudslides, Minxian,Yushu and Wenchuan disaster area. The project put forward the hypothesis, which the intense trauma PTSD sleep disorders occur stress leads to abnormal neuronal injury of hippocampus, temporal characteristic and the code mechanism, resulting in sleep regulation dysfunction, the endemic species in Loess Plateau has the typical circadian rhythm of Myospalax cansus copy PTSD animal model, evaluation of zokor abnormal sleep EEG phase and feature detection; This project is based on the relevant research and the hippocampus neural coding theory and modelby, using the body multi-channel microelectrode array technology(M-NEMEA), the change of hippocampal (CA1、CA3) neurons space-time mode and encoding features of PTSD sleep disorders is detected; combining with related Chinese medicine theory and past work foundation, to exploreand reveal the mechanism of Sini San to space and time characteristics, information transfer, integration mechanisms and functional remodeling of hippocampal neurons of PTSD Myospalax cansus accompanying sleep disorders, which will provide the basis for mechanism research and applicati on medicine prevention of PTSD and sleep disorders.

英文关键词： Myospalax cansus;PTSD;Sleep Disorders;Sini San;Hippocampus Neural Coding Mechanism