Periostin促进骨质疏松症牙周组织再生修复作用的研究

项目名称： Periostin促进骨质疏松症牙周组织再生修复作用的研究

项目编号： No.81470774

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 丁寅

作者单位： 中国人民解放军第四军医大学

项目金额： 73万元

中文摘要： 雌激素缺乏是绝经后妇女骨质疏松的重要原因，常导致牙槽骨吸收加快，牙周组织的再生与修复困难。Periostin是维持牙周组织结构和功能完整最为重要的细胞外基质蛋白之一。新近研究发现，雌激素可能通过调控Periostin 参与调节牙周组织的代谢和功能改建。我们前期研究证实，去势大鼠BMSCs功能异常，增殖和成骨分化能力下降，Periostin表达减少，可能是导致骨质疏松和牙周骨缺损的重要原因。为此，本项目将以BMSCs为研究核心，借助携带Periostin 基因的慢病毒转染去势大鼠BMSCs，观察上调Periostin表达对BMSCs生物学行为的影响，并通过信号干涉、生物芯片及RNA干扰等深入研究其调控机制；同时构建基于细胞膜片/多肽纳米纤维仿生细胞外基质的新型细胞复合体，并在此基础上进行牙周组织缺损的修复实验，从而为绝经后骨质疏松症患者牙周病的干细胞移植治疗开辟一条新的途径。

中文关键词： 骨质疏松；雌激素；牙周再生；细胞膜片；细胞外基质蛋白

英文摘要： Estrogenic deficiency is an important risk factor for postmenopausal osteoporosis, which may lead to accelerated alveolar bone resorption and difficult regeneration and reconstruction of periodontal tissues.It has been indicated that Periostin is one of the most important extracellular matrix proteins in maintaining the structural and functional integrity of periodontal tissues.According to a recent study, estrogen might participate in the regulation of periodontal metabolism and reconstruction via regulating Periostin. Our prelimitary data have shown that BMSCs from ovariectomized rats is functionally disturbed with discreased proliferation and osteogenic differentiation capacities and down-regulated expression of periostin, which might be the leading reason for osteoporosis and periodontal bone loss.In this project, lentivirus harboring the periostin gene will be used to transfect the BMSCs from ovariectomized rats and the changes of biological behavior of BMSCs will be observed.A further regulatory mechanism will be investigated by pathway interference,detection of biochips and siRNA. Meanwhile, a novel cell aggregates based on cell sheet engineering and self-assembling peptide nanofiber scaffold will be created and a repair experiment for periodontal defect will be performed. Our project will develop a new approach for the treatment of periodontal disease combined with postmenopausal osteoporosis based on MSCs transplantation.

英文关键词： osteoporosis;estrogen;periodontal regeneration;cell sheet;extracellular matrix