初级纤毛介导的Shh信号与小鼠腭发育关系的研究

项目名称： 初级纤毛介导的Shh信号与小鼠腭发育关系的研究

项目编号： No.81360165

项目类型： 地区科学基金项目

立项/批准年度： 2014

项目学科： 医药、卫生

项目作者： 何苇

作者单位： 遵义医科大学

项目金额： 48万元

中文摘要： Shh信号通路在腭发育中有重要作用，但其下游信号如何传递尚不清楚。研究证实哺乳动物多种组织中纤毛可传递Shh等信号因子，同时参与调控细胞周期，也可对外环境的改变发生相应变化。申请者前期研究发现地塞米松和维生素B12作用于特定孕期母鼠后，胎鼠腭板Shh信号表达和间充质细胞增殖均发生改变，基于这一发现，申请者假设小鼠腭胚突间充质细胞Shh信号传导依赖初级纤毛，外界环境因素可通过影响纤毛蛋白组件调控Shh下游信号因子传递，二者协同调节间充质细胞周期，从而影响腭的发育。故本课题利用细胞体外培养系统，运用免疫组织化学、扫描电镜、激光共聚焦显微镜和RNAi等技术检测Shh信号在小鼠腭胚突间充质细胞中的传递模式，观察外界因素干预下，如地塞米松等对Shh信号传递、细胞周期和纤毛动态变化的影响，探讨它们之间的关系，从一个新的视角研究环境因素影响腭发育的机制。

中文关键词： 初级纤毛;腭发育;信号通路;;

英文摘要： Shh signaling play a important role during palatal development,but the transduction of of Shh downstream signaling remained unknown.Studies showed transduction of Shh signaling dependent on primary cilia in some kinds of tissue in mammalian and primary lilia involved in regulating cell cycle and response to environment.Applicant discovered that expression of Shh signaling and proliferation of mesenchyme at mouse embryonic palate was changed after timed pregnant mice was treated with DEX and VitB12.Applicant make a hypothesis that transduction of Shh signaling in mesenchyme of mouse embryonic palate dependent on primary cilia. That Environment factors regulate transduction of Shh signaling by effection ciliary components, which control mesenchymal cells cycle. So the object of the study is to detect the pattern of Shh signaling transduction in mouse palatal embryonic cells and change of environment factors,such as DEX, make effect on Shh signaling transduction,cells cycle and dynamic change of cilia by immunohistochemistry, scanning electron microscope, LSCM, RNAi, and so on. To explore the mechanism of evironment factors influence palatal development from a new perspective.

英文关键词： primary cilia;palatal development;signaling pathway