中介体亚基TaMed25调控小麦白粉病抗性的分子机理研究

项目名称： 中介体亚基TaMed25调控小麦白粉病抗性的分子机理研究

项目编号： No.31501297

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 食品科学、农学基础与作物学

项目作者： 刘杰

作者单位： 中国农业科学院作物科学研究所

项目金额： 20万元

中文摘要： 小麦是世界上和我国最主要的粮食作物之一，提高小麦产量对保证我国粮食安全和可持续发展意义重大。白粉病是我国小麦生产中的三大病害之一，导致小麦的大量减产，因此研究小麦抗白粉病的分子调控机理将有助于我们培育对白粉病具有持久、广谱抗性的小麦新品种，为我国粮食高产、稳产做出贡献。植物中介体是基因转录事件的重要调节因子，在植物抗病反应调控中发挥重要作用，但目前国内外还未见关于小麦中介体亚基参与小麦抗病的分子机制研究。我们从分析中介体亚基TaMed25调控小麦白粉病抗性的生物学功能入手，通过实验证明它能够与乙烯信号调控的关键因子TaEIL1直接物理互作。进一步研究表明，TaMed25与TaEIL1的互作与小麦的白粉病抗性反应密切相关。本项目将利用分子生物学、遗传学等方法，进一步解析中介体与乙烯信号互作调控小麦白粉病抗性的分子调控机制。本项目的开展将为小麦抗病分子育种提供重要的理论依据。

中文关键词： 小麦；白粉病；病原菌-寄主互作

英文摘要： Bread wheat (Triticum aestivum L.) is one of the main food crops in our country, thus, improvement of wheat grain yield is of vital importance for the national food security and agricultural sustainable development. Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici (Bgt), is one of the most damaging wheat diseases worldwide, leading to severe yield reduction of wheat. To improve wheat disease resistance to powdery mildew, it is crucial to explore the regulatory mechanisms associated with wheat immune responses to powdery mildew fungus. The plant multiprotein Mediator complex is recognized as the core transcriptional regulation component, and plays a critical role in plant immune responses. In this study, we focus on the function of Mediator subunit TaMed25 in the regulation of wheat disease resistance against Bgt infection. We performed bimolecular fluorescence complementation assay and luciferase complementation imaging assay, and proved that wheat TaMed25 physically interacts with TaEIL1, the key transcription factor in ethylene signaling. We propose to explore the regulation roles of TaMed25 in ethylene signaling and further to elucidate its action mechanism in wheat immune responses to Bgt infection. Our work will be valuable and in great need for wheat disease-resistance breeding.

英文关键词： Wheat;Powdery mildew;Microbe-host interaction