Cry1Ah蛋白杀虫特异性分子机制

项目名称： Cry1Ah蛋白杀虫特异性分子机制

项目编号： No.31272115

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 张杰

作者单位： 中国农业科学院植物保护研究所

项目金额： 85万元

中文摘要： 目前全球发现的cry基因已达622种，但由于毒力有限或杀虫谱窄等原因，大部分cry基因无法直接应用。Cry1Ah是本实验室克隆的具有自主知识产权的新型杀虫基因，其编码的蛋白对多种鳞翅目害虫具有很高的杀虫活性，且对家蚕毒性很低，目前转cry1Ah基因水稻和玉米已经进入田间试验阶段，成为我国新一代抗虫转基因作物创制的保证。本项目拟以结构相似、杀虫活性"相反"的Cry1Ah和Cry1Ai蛋白为研究材料，分析比较两者与昆虫BBMV的互作情况，寻找昆虫BBMV上特异性结合蛋白，明确Cry蛋白与杀虫特异性相关的Loop区域氨基酸组成，揭示Cry1Ah蛋白对棉铃虫等农业害虫高毒力、对经济昆虫家蚕相对安全低毒的分子机制。这项研究瞄准国家重大需求，不仅从理论层面揭示Cry1Ah蛋白的杀虫特异性机制，为cry1Ah基因的推广应用奠定坚实的理论基础，同时也为其它cry基因的高效、安全分子设计提供理论指导。

中文关键词： Cry1Ah；杀虫特异性；氨肽酶N；棉铃虫；家蚕

英文摘要： There are 622 cry genes cloned in the world now, but most of them can't be utilized directly because of the low toxicity or narrow insecticidal spectrum. The novel gene cry1Ah, intellectual property rights owned, was cloned by our lab, it encoded a protein exhibited a strong toxicity against many lepidopteran insects, except Bombyx mori. The transgenic maize and rice carrying the cry1Ah gene have been assessed in field trial stage, and it will become the important guarantee for the creation of the new generation biotechnological crops. In this proposal, we are planning to analyze interaction between Cry1A protein and BBMV from insect midgut, and binding difference between Cry1Ah and Cry1Ai, which shares the similar structure but contrary function with Cry1Ah protein, will be investigated. To search the specific binding protein in BBMV, to explore the Loop region of specific insecticidal activity from Cry1Ah/Cry1Ai protein, and to reveal the molecular mechanism of Cry1Ah with strong toxicity against many agricultural insect pests such as Helicoverpa armigera, and safe to economic insect Bombyx mori,will be conducted furthermore.In this proposal, the national significant demand is being aimed, the mechanism of insecticidal specificity of Cry1Ah will be elucidated. All of these research progress will lay the founda

英文关键词： Cry1Ah；insecticidal specificity；aminopeptidase N；Helicoverpa armigera；Bombyx mori