兰州地区呼吸道合胞病毒流行特征的研究

项目名称： 兰州地区呼吸道合胞病毒流行特征的研究

项目编号： No.81260001

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 医药、卫生

项目作者： 张蓉芳

作者单位： 甘肃省妇幼保健院

项目金额： 49万元

中文摘要： 呼吸道合胞病毒是导致儿童急性呼吸道感染,尤其是儿童严重下呼吸道感染的最主要病原之一。申请人前期研究工作证实兰州地区呼吸道感染患儿中病毒感染率很高(73.92%)，其中RSV占据首位,感染率达(38.14%).持续、深入地开展RSV的流行病学研究将对该疾病的预防控制具有重要的现实意义。本研究拟采用RT－PCR和PCR方法连续筛查兰州地区急性呼吸道感染住院儿童中的14种呼吸道病毒，并建立呼吸道合胞病毒G蛋白基因全长扩增方法，旨在全面了解兰州地区儿童急性呼吸道感染病毒病原谱的基础上，重点分析呼吸道合胞病毒感染的流行病学特征，深入研究呼吸道合胞病毒G蛋白基因的进化变异规律，为RSV疫苗的研制及本地区该疾病的预防控制提供基础数据。

中文关键词： 呼吸道合胞病毒;儿童;呼吸道感染;G蛋白;聚合酶链式反应

英文摘要： Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections among infants and young children，especially a major cause of morbidity in children under 1 year of age. The previous researches of the present project reveal that respiratory viruses lead to high rates of infection in children in Lanzhou, and RSV was the most frequently detected respiratory virus in children with ALRTIs in our study. Therefore it is of great significant to make further and deep studies of RSV for better prevention and control of the disease.The present project will apply PCR and RT-PCR to screen the 14 viruses RSV, HRV, IFVA, IFVB, AdV, PIV1-3, and the newly indentified viruses HCoV-HKU1, HCoV-NL63, hMPV, HBoV1-3 in hospitalized children in Lanzhou. The project plans to work out the method to amplify the full length of G protein of respiratory syncytial virus. On the basis of the knowledge of pathogen spectrum in lanzhou area, our study aims to analyze epidemiological characteristics of RSV and the mode of evolution and variability of the G protein so as to provide data for further researches of vaccines as well as the prevention and control of the disease in our region.

英文关键词： respiratory syncytial virus;children;respiratory tract infection;G Glycoprotein;PCR