分子识别功能纳米核壳组装体构造及其金属增强荧光效应

项目名称： 分子识别功能纳米核壳组装体构造及其金属增强荧光效应

项目编号： No.21505103

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 顾雪凡

作者单位： 西安石油大学

项目金额： 21万元

中文摘要： 金属增强荧光（MEF）是一种荧光光物理过程信号放大途径。项目组前期研究表明制备不同贵金属纳米粒子，调控其形成组装体构造子间距，有利于调控组装体局域表面等离子体共振（LSPR）效应。本项目基于时域有限差分法（FDTD）设计、制备球形AuNPs、球形AgNPs、具有不同LSPR三角形AgNPs（triAgNPs）；分别包覆聚(N-异丙基丙烯酰胺)（PNIPAM）形成温敏核-壳纳米组装体，采用动态光散射技术和紫外-可见吸收光谱研究其水溶液稳定性；采用透射电子显微镜研究其微观形貌；采用荧光光谱研究其壳层环境响应性。通过荧光标记双链DNA（dsDNA）介导形成AuNPs/AgNPs@PNIPAM/PFV二聚体，由DNA和PNIPAM协同调节距离，获得既定耦合LSPR效应；通过荧光发射光谱测定和荧光寿命分析证实该体系具有显著的MEF效应以及可调控的stokes位移，建立ATP的高灵敏检测新方法。

中文关键词： 金属增强荧光；局域表面等离子体共振；金属纳米粒子；组装体；聚(N-异丙基丙烯酰胺)

英文摘要： Metal-enhanced fluorescence (MEF) is a signal amplification pathway for photophysical process of fluorescence. The results in our previous studies demonstrated that it was beneficial for noble metal nanoparticles assemblies to have different constructors and the appropriate distance between them in order to get various localized surface plasmons resonance (LSPR). In this proposal, based on the results of finite difference time domain (FDTD), we designed and prepared AuNPs, AgNPs and triAgNPs with different LSPR, and make them coated with poly (N- isopropylacrylamide) (PNIPAM) forming a thermo-sensitive core-shell nano-assembly, respectively. Their stability in aqueous solutions will be measured by dynamic light scattering and ultraviolet-visible absorption spectra, their morphology will be observed via transmission electron microscopy, and their thermo-sensitivity will be studied by fluorescence spectra. Then, AuNPs/AgNPs@PNIPAM/PFV dimer will be prepared by fluorescent molecules tagged double-stranded DNA (dsDNA) and LSPR coupling effect will be obtained by regulating the distance with synergistic effect of DNA and PNIPAM; their significant MEF effect and stokes shift will be measured by fluorescence spectra and fluorescence lifetime, ultrasensitive method for detecting ATP will be developed.

英文关键词： Metal-enhanced fluorescence (MEF);Localized surface plasmons resonance(LSPR);Metal nanoparticles;Assemly;Poly (N- isopropylacrylamide) (PNIPAM)