壳聚糖季铵盐基复合膜改性钛涂层的制备及抗感染性能研究

项目名称： 壳聚糖季铵盐基复合膜改性钛涂层的制备及抗感染性能研究

项目编号： No.81501856

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 敖海勇

作者单位： 华东交通大学

项目金额： 18万元

中文摘要： 感染是骨科植入物术后最严重的并发症之一，通过骨科植入物材料表面的改性，防止细菌的黏附和生物膜形成，有望达到预防感染发生的目的。前期研究中我们已通过对壳聚糖的改性，研制出抗菌性能更强的壳聚糖季铵盐材料，能够抑制临床常见致病菌包括耐药菌的生长；对骨源性细胞也有良好的生物相容性。在前期工作中，我们还制备了结构稳定、骨整合性能良好的I型胶原-透明质酸复合膜改性钛涂层。本项目拟将壳聚糖季铵盐引入到复合膜中，利用层-层共价键接枝法制备I型胶原-透明质酸-壳聚糖季铵盐复合膜改性钛涂层。优化工艺参数，考察复合膜改性钛涂层的表面性能、机械和化学稳定性。通过体外生物学和体内动物实验，考察其抑制细菌粘附和生物膜形成，预防感染发生的能力以及骨整合效果，并分析抗菌机理。预期将开发出同时具有良好抗感染性能和骨整合能力的新型骨科植入物材料，具有较好的临床需求和应用前景。

中文关键词： 壳聚糖季铵盐；生物大分子复合膜；钛涂层；层-层共价键接枝法；抗感染性能

英文摘要： Infection is one of the most serious complications of orthopedic implant surgery. Surface modification of orthopaedic implant materials to prevent bacterial adhesion and biofilm formation is expected to reach the purpose of preventing infection occurred. In our previous study, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) with better anti-bacteria property has been prepared through modification of chitosan. HACC can inhibit the adhesion and biofilm formation of clinical common pathogenic bacteria including drug-resistant bacteria, and possess good compatibility to osteocyte. We also prepared collagen I-hyaluronic acid multilayer modified titanium coating with good structure stability and biological properties. In this project, HACC is introduced into the composite membrane, and collagen I-hyaluronic acid-HACC composite membrane modified titanium coating is prepared by layer-by-layer (LBL) covalent immobilization. Process parameters are optimized. Surface properties, mechanical and chemical stability of composite membrane modified titanium coating are studied. With in vitro and in vivo study, ability of inhibiting bacterial adhesion and biofilm formation is investigated as well as the capacity of preventing infection. Moreover, mechanism of antibacterial is analyzed. It’s expected that a novel orthopaedic implant material with excellent anti-infective property and osseointegration performance will be developed, which has extensive clinical requirement and good application prospect.

英文关键词： hydroxypropyltrimethyl ammonium chloride chitosan;biomacromolecule composite membrane;titanium coating;layer-by-layer covalent immobilization;anti-infective property