棉花GhGolS1基因在花药和棉纤维次生壁发育中的功能研究

项目名称： 棉花GhGolS1基因在花药和棉纤维次生壁发育中的功能研究

项目编号： No.31201252

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 周焘

作者单位： 中国农业科学院生物技术研究所

项目金额： 23万元

中文摘要： 我们从棉花生殖发育相关基因簇中克隆了GhGolS1，其是调控棉子糖半乳糖苷系列寡糖RFOs代谢的限速酶，初步研究发现该基因并非像前人发现的那样参与抗性生理、种子生理或光合产物运输，而是与花药和纤维次生壁发育相关，且该基因与基因簇中GhAPm和GhNeuF的排列结构在进化中非常保守，因此本项目拟重点研究GhGolS1，检测该基因的过表达和RNAi干扰转基因棉花花药发育、纤维品质和农艺性状；通过荧光实时定量PCR和原位杂交了解该基因在转基因材料和棉花CMS三系材料中的表达情况，用体式显微镜、扫描电镜和高效液相色谱检测因该基因上下调引起的花药、花粉和纤维结构及RFOs代谢途径的变化，通过双分子荧光互补、酵母双杂交和荧光实时定量PCR验证该基因与GhAPm和GhNeuF的关系。阐明GhGolS1在花药和纤维次生壁发育过程中的分子机理，揭示该基因与其它基因的互作关系，为研究整个基因簇功能打下基础。

中文关键词： 棉花；肌醇半乳糖苷酶基因；花药；纤维；发育

英文摘要： We had cloned GhGolS1 from a cotton gene cluster involved in cotton reproduction development, the gene encodes galactinol synthase and it is the key enzyme in raffinose family oligosaccharides (RFOs) metabolic pathway. Preliminary studying found the function of GhGolS1 is different from other people's research result, it's not involved in resistance physiology, seed physiology or photosynthate translocation, but involved in anther and the secondary cell wall of fiber development, and the result of bioinformatics analysis reveal the gene structure of GhGolS1, GhAPm and GhNeuF is very conserved in different plant. Based on the above reasons, we plan thorough research GhGolS1. first of all, we will detect anther development, fiber quality and other important agronomic traits of GhGolS1 transgenic overexpress cotton and RNAi cotton; secondly, we will study the GhGolS1 transcription in the transgenic lines and the cotton CMS three lines by the real-time PCR and northern blot in situ hybridization; thirdly, we will use stereomicroscope, scanning electron microscope and high performance liquid chromatography to detect the change of structure of relative tissue and cell and RFOs metabolic pathway resulted by GhGolS1 up-regulated and down-regulated; finally, we will identify the interaction relation between GhGolS1,GhAPm

英文关键词： cotton；GhGolS1；anther；fiber；development