热应激下肉鸡固有免疫应答及白藜芦醇调控的分子机制

项目名称： 热应激下肉鸡固有免疫应答及白藜芦醇调控的分子机制

项目编号： No.31672444

项目类型： 面上项目

立项/批准年度： 2017

项目学科： 畜牧学与草地科学

项目作者： 贺建华

作者单位： 湖南农业大学

项目金额： 25万元

中文摘要： 目前鸡对热应激反应不同组织器官中报道的差异基因不一致，高温下鸡自我免疫调控机制需不断完善。我们前期研究发现白藜芦醇能降低热应激下肉鸡肠道炎症损伤，但白藜芦醇缓解鸡热应激的作用机制仍不清楚。本研究建立仔鸡热应激模型，运用荧光定量PCR、免疫印迹、流式细胞术等技术，重点探讨热应激下鸡脾脏内涉及免疫反应的差异基因、蛋白与信号通路，阐明白藜芦醇对热应激下鸡固有免疫应答的调控效果，解析白藜芦醇调控热应激下鸡免疫反应的靶基因、蛋白及信号通路。本研究从基因、蛋白、细胞水平与信号通路角度阐释热应激下鸡固有免疫应答及白藜芦醇调控的分子机制，将为家禽高温环境下自我免疫调控机制及白藜芦醇生物学功能研究提供理论依据。

中文关键词： 鸡；白藜芦醇；热应激；营养免疫；分子营养

英文摘要： At present, differential genes reported were not consistent in the different tissues and organs of chicken under heat stress, so the immune modulation mechanism of chicken under high temperature still need to be explored. Our previous study found that resveratrol could reduce the intestinal inflammatory damage of broiler under heat stress, but the mechanism of resveratrol in alleviating chicken heat stress is still unclear. Therefore, heat stress model with chicken will be established in this study, the differential genes, proteins and signal pathway in innate immune responses in the spleen of chicken with heat stress will be explored by fluorescence quantitative PCR, Western blot, gel migration, flow cytometry, et al., and the regulation effect of resveratrol on the innate immune response of chicken under heat stress will be analyzed and deduced. Thereafter, the target genes, proteins and signal pathways of resveratrol regulating on the innate immune responses in chicken under heat stress will be explored, and the regulation mechanism of resveratrol in chicken under heat stress will be deduced in this study from the view of target gene, protein and cell level and signal pathway, the above results will provide a theoretical basis for the self immune regulation mechanism of poultry under the high temperature environment and the biological function study of resveratrol.

英文关键词： Broiler;Resveratrol;Heat stress;Nutritional immunity;Molecular nutrition