LncRNA-LINC01133作用机制以及在胆囊癌中功能研究

项目名称： LncRNA-LINC01133作用机制以及在胆囊癌中功能研究

项目编号： No.91440203

项目类型： 重大研究计划

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 刘颖斌

作者单位： 上海交通大学

项目金额： 300万元

中文摘要： 长链非编码RNA（lncRNA）具有调控基因表达的重要作用，在肿瘤研究中展现了极大的研究前景。胆囊癌相关研究仍未深入进行，我们在前期研究中经过lncRNA芯片筛选并锁定了一条在胆囊癌中可能具有重要生物学功能的lncRNA-LINC01133，功能学研究提示其可调控胆囊癌细胞增殖以及转移能力，进一步利用表达谱芯片、生物信息学分析以及双荧光素酶报告实验等方法，其可能通过抑制邻近基因表达以及与多个微小RNA（miRNA）相结合的方式来发挥生物学功能。在本项目中，我们将从与蛋白结合、与miRNA、以及与RNA（非miRNA）相互作用等角度研究LINC01133的作用机制，勾勒出LINC01133下游调控网络，同时我们还拟对LINC01133进行结构鉴定、生物学活性研究、以及其在基因信息传递过程中的机制研究，并判断其在胆囊癌发生发展过程的重要意义。

中文关键词： 长链非编码RNA；竞争性内源RNA；胆囊癌；分子机制；基因调控

英文摘要： Long non-coding RNA (lncRNA) plays an important role in regulation of gene expression, and thus has attracted a lot of attention, especially in the field of cancer research. However, its relevant research in gallbladder cancer has just begun. In our previous work, we have identified a novel lncRNA-LINC01133, which might has a biological effect in gallbladder cancer, by lncRNA microarray. The function study has demonstrated that LINC01133 promotes proliferation and invasion of gallbladder cancer cells. In addition, the further gene microarray and bioformatic analysis has identified that LINC01133 plays its biological role via downregulating nearby genes and combining miRNA. In this study, we plan to do the structure identification, function and mechanism research on LINC01133, and demonstrate its role in gallbladder cancer progress. To explore its mechanism, we plan to conduct the research from three perspectives, which include protein, miRNA and mRNA. Hopefully, the downstream regulation network could be clarified by this study.

英文关键词： long non-coding RNA;ceRNA;Gallbladder cancer;molecular mechanism;regulation of gene expression