典型封闭型海湾生境滩涂沉积物微生物群落结构及功能对沙蚕生态修复的响应

项目名称： 典型封闭型海湾生境滩涂沉积物微生物群落结构及功能对沙蚕生态修复的响应

项目编号： No.31272669

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 马英

作者单位： 集美大学

项目金额： 68万元

中文摘要： 三沙湾是我国典型封闭型海湾，全国唯一内湾性大黄鱼产卵场及最大海水网箱养殖基地。三沙湾还承纳着周边200多万人口生产生活污水，环境污染问题日趋严重。传统的环境状况评估多采用物理、化学指标和大型动植物，及肉眼可见的小型底栖生物。而更能灵敏表征环境状况改变的微生物，由于检测手段的限制及人们对其知之甚少而未被采用。本项目从在生态系统结构和功能上起重要作用的微生物入手，借助本单位正承担相关海洋公益项目的便利条件，用构建克隆文库、DGGE、Southern杂交、biolog、高通量测序等手段，研究海湾生境沙蚕生态修复过程沉积物中活性微生物的群落结构、功能及动态变化，分析其与环境因子和修复行为间的关系，研究可灵敏反映环境状况变化的微生物参数，以全新数据和视角审视典型封闭型海湾生境环境状况。为优化修复措施、完善环评指标奠定基础，并为近岸海域生态系统结构功能、生源要素的生物地球化学循环及污染物的转化提供数据

中文关键词： 封闭型海湾；滩涂沉积物；微生物；生物修复；响应

英文摘要： Sansha bay is a typical enclosed bay in China. It is the only inner bay for large yellow croaker spawning and the largest marine cage breeding base in China. Sansha bay also carries production sewage and living sewage of over 200 million people. Thus the problem of environmental pollution is increasing. Traditionally, the environmental quality is assessed by physical, chemical indicators or a number of large plants and animals, and visible small benthic organisms. Although microorganisms are more sensitive to environmental changes and are better indicators for environmental quality assessment, they have not been adopted due to the limit of detection means and people's poor understanding for them. One ongoing Ocean Public Welfare Research Project in our college is to restore the polluted mudflat sediments in Sansha bay using clamworm. With the convenience of sampling and data sharing of the ongoing project, and from the point of view of microbes, which play an important role in gulf ecosystem, the proposed project here aims to follow the microbial community structures and functions and their dynamics during the bioremediation through a combined approach involving clone libraries constructions, denaturing gradient gel electrophoresis(DGGE), Southern blot，biolog and high-throughput sequencing, etc. The relationship

英文关键词： enclosed bay；intertidal sediment；microorganism；bioremediation；response