血管组织工程用可降解磷脂聚氨酯支架及其对细胞行为的影响

项目名称： 血管组织工程用可降解磷脂聚氨酯支架及其对细胞行为的影响

项目编号： No.51273124

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 一般工业技术

项目作者： 李洁华

作者单位： 四川大学

项目金额： 79万元

中文摘要： 旨在揭示磷脂聚合物在血管组织再生过程中所起的作用，特别是对在血管组织再生中起重要作用的免疫细胞的影响。本课题以具有优异抗凝血性能，能大大提高人工血管通畅率的磷脂聚合物为研究对象,设计、制备可完全生物降解的磷脂聚氨酯，将其作为表面改性大分子与水性可降解聚氨酯共混，用冷冻干燥法制备三维多孔支架，通过调节各组分比例、溶液浓度、制备工艺等控制支架的孔结构、力学性能及降解速度，获得具有优异抗凝血性能，与天然血管相近的力学顺应性，降解速率可调控的人工血管组织工程支架。采用体外细胞培养分别研究磷脂聚氨酯支架及其降解产物对单核/巨噬细胞表型的影响，对内皮细胞和平滑肌细胞生长的影响，以及细胞对支架降解的影响。并通过细胞共培养，研究支架上不同功能细胞之间的相互作用。探讨支架中磷脂及其含量对血管组织再生功能细胞的影响，尤其是对单核/巨噬细胞行为的影响，为小血管组织工程支架的设计提供有益的理论基础和科学依据。

中文关键词： 聚氨酯；磷脂；组织工程支架；可降解；软组织修复

英文摘要： To reveal the role of phospholipid polymer in regeneration of vascular tissues, especially, the effects on immune cells that play a key role in the regeneration, phospholipid polyurethanes will herein be employed to investigate their influences on blood immune cells, which can largely improve patency of synthetic vascular grafts because of their excellent hemocompatibility. Thus, novel biodegradable phospholipid polyurethanes as surface modifying macromolecules will be designed, synthesized, and characterized. These phospholipid polyurethanes will be blended into waterborne biodegadable polyurethanes for vascular tissue engineering scaffolds. A series of three-dimensional interconnected porous scaffolds will be fabricated using the biodegradable phospholipid polyurethane blends by freeze-drying. Their properties, such as pore size, antithrombogenicity, matching with native vessel compliance, biodegradable rate, and mechanical properties, will be controlled by varying the ratios of the phospholipid polyurethane and waterborne biodegradable polyurethane in these blends, concentrations of these blends emulsions and process to obtain optimal scaffolds of these phospholipid polyurethane blends. The interaction between cells and these obtained scaffolds will be comprehensively studied in vitro, including monocyte-der

英文关键词： polyurethanes；phospholipid；scaffolds；biodegradable；soft tissue repair