小直径血管支架材料Co-Cr-Cu合金的制备及其对血管再狭窄的抑制作用

项目名称： 小直径血管支架材料Co-Cr-Cu合金的制备及其对血管再狭窄的抑制作用

项目编号： No.31470930

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 张二林

作者单位： 东北大学

项目金额： 85万元

中文摘要： 在支架介入治疗小直径血管狭窄中，不可降解支架存在血管再狭窄和晚期血栓问题，而完全可降解支架又存在支撑力不足的问题，因此，迫切需要一种全面的解决方案。本申请基于Cu离子具有促进内皮细胞生长和抑制平滑肌细胞生长的作用，在Co-Cr中添加Cu元素，制备Co-Cr-Cu合金。通过调整Cu元素的存在形式、尺寸和分布等组织特征及含量，控制Cu离子的溶出，实现促进内皮化和抑制平滑肌增生、抑制再狭窄和晚期血栓，同时合金又具有足够的支撑力。重点研究Cu元素组织特征和含量对Cu离子溶出行为的影响规律、Cu离子对内皮细胞和平滑肌细胞生长特性的影响规律，揭示其作用机理，建立合金中Cu元素组织特征和含量与合金表面内皮细胞和平滑肌细胞的生长特性、合金力学性能、耐蚀性能和血液相容性间的关联关系，最后体内实验评价Co-Cr-Cu支架对血管再狭窄和晚期血栓形成的抑制作用，为Co-Cr-Cu支架材料的发展提供实验依据和理论。

中文关键词： 生物医用材料；生物相容性；支架材料；Co合金；再狭窄

英文摘要： In the stent interventional treatment,for example, percutaneous transluminal coronary angioplasty(PTCA), the in-stent restenosis (ISR) and the late stent thrombosis(LST) are still serious clinical problems for the non-degradable stent, and on the other hand the mechanical properties of the biodegradable stent are not strong enough, especially in the case of blood vessels with small diameter. Therefore, it is very necessary to find out a new solution to resolve the problems. In this proposal, Cu element will be added into Cr-Co alloy to prepare Co-Cr-Cu stent material based on the knowledge that Cu ion stimulates proliferation of vascular endothelial cell (VEC) and inhibits the proliferation of smooth muscle cell (SMC). It is proposed that the Cu ion released from the alloy will promote the endothelialization and restrain the proliferation of SMC, which results in the preventing of ISR and LST. It is believed that the Cu ion release will be controlled by changing the microstructure characteristic of Cu element, such as existence form, size and distribution, and content.The research will mainly focus on the effect of the microstructure characteristic of Cu element on the Cu ion release behavior. The influence of Cu ion on the adhesion and growth characteristics of VEC and SMC cells will be investigated in detial and the controlling mechanism will be discussed. A relationship will be put forward between the microstructure characteristics of Cu element and the adhesion and proliferation characteristics of both cells, mechanical properties and corrosion property of Co-Cr-Cu alloy and blood compatibility. Finally, in-vivo experiments will be carried out to evaluate the preventing function of the stent made of Co-Cr-Cu alloy from ISR and LST. All these will provide us with practical results and theoretical guide for the development of Co-Cr-Cu stent materials.

英文关键词： biomaterials;biocompatibility;stent materials;Co alloy;restenosis