麻黄-杏仁药对减轻气道损伤的配伍机理研究

项目名称： 麻黄-杏仁药对减轻气道损伤的配伍机理研究

项目编号： No.81460610

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李文宏

作者单位： 江西中医药大学

项目金额： 48万元

中文摘要： 哮喘是四大顽症之一，糖皮质激素仍然是疗喘的首选。新近研究表明，哮喘的严重程度与气道上皮的损伤范围、EGFR介导的修复功能缺失程度成正比，因此，以开发具有上皮保护作用的药物是今后研究的新方向之一。中医药治喘历史悠久、疗效可靠，疗喘方运用最多的药味是麻黄、杏仁。从成分和效应之间的关系阐释配伍规律是复方研究的重要领域，药对由于药简力宏是主要研究对象。前期研究发现：麻杏石甘汤可明显减轻气道损伤，主要药味是麻黄、杏仁（最佳剂量配比4：5），但其内涵尚不清楚。本研究以麻黄-杏仁药对为对象，应用含药肠吸收液方法，采用RRLC-QTOF/MS分析配伍的成分变化；HCS、RT-PCR技术和Pearson分析，多层次探讨其抗气道损伤与EGFR/PI3K通路关键元件的相关性；并经主成分分析，初步阐释其配伍-成分-效应之间的规律。深化麻黄-杏仁药对的认识，为配伍研究提供示范和新的思路，促进复方的现代阐释。

中文关键词： 中药药对；配伍机制；药效物质基础；分子机制

英文摘要： Asthma is one of the four chronic and stubborn disease, Glucocorticoid is still the preferred treatment of asthma. Recent studies showed that the severity of asthma is proportional to the scope of airway epithelium damage and the degree of deficiency repair functions mediated by EGFR, Therefore, the drug to develop epithelial protective effect is one of the new research direction in the future. Traditional Chinese medicine has a long history of treating asthma, the curative effect is reliable. The most used medicine of treating asthma decoction is ephedra and almond. From interpreting the relationship between compatibility rules between components and effect is an important field of research due to the compound, Couplet medicines is the main object of study because it use a small number of medicine but strong pharmacological action. Previous study found that: Maxingshigan Decoction significantly reduced airway injury, the main ingredients are ephedra, almond (optimal dose ratio is 4:5), but its scientific connotation is not clear. In this study, couplet medicines of ephedra and almond was studied as the object, pharmacological methods of the bowel absorption liquid containing medicine composition is used. Technology of RRLC-QTOF/MS was used to analysis the chemical changes in compatibility. Technology of HCS, RT-PCR and Pearson analysis were used to investigate the correlation between anti airway injury and the key components of the EGFR/PI3K pathway multi-level. By using principal component analysis, to preliminary explane the scientific connotation of the regular pattern among compatibility, ingredients and effect. Through the study, we want to deepen the understanding of couplet medicines of ephedra and almond, provide demonstration and new ideas for the research on the compatibility, promote the modern interpretation of Chinese compound.

英文关键词： Couplet Medicines;Mechanism of Composition;Pharmacodynamic Substance Foundation;Molecular Mechanism