缺氧和福美双对藏鸡和肉鸡胫骨生长板HIF-1α、Hsp90及VEGF/VEGF受体表达的影响

项目名称： 缺氧和福美双对藏鸡和肉鸡胫骨生长板HIF-1α、Hsp90及VEGF/VEGF受体表达的影响

项目编号： No.31460682

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 农业科学

项目作者： 李家奎

作者单位： 西藏农牧学院

项目金额： 50万元

中文摘要： 缺氧致哺乳动物HIF-1α表达水平增高，诱导血管生成相关因子Hsp90、VEGF生成。肉鸡胫骨软骨发育不良（TD）是由于病变部位血管发育障碍，缺氧是其中的一个关键因子。我们前期的研究发现，Hsp90在肉鸡TD时其胫骨生长板表达水平增加，但却并不能促进血管生成，其中的机理需要进一步研究。本项目以藏鸡为比较模型，研究藏鸡和肉鸡在缺氧环境条件和福美双诱导TD时胫骨生长板HIF-1α、Hsp90和血管生长相关因子bFGF、VEGF、及其受体等的时序性表达差异；结合细胞培养实验，研究藏鸡和肉鸡胫骨生长板软骨细胞在缺氧和福美双刺激条件下细胞分化、HIF-1α、Hsp90、bFGF、VEGF及其受体表达的特点，观察藏鸡和肉鸡在缺氧和福美双诱导下胫骨生长板血管生成的差异，探究品种影响胫骨生长板血管生成的差异的机制。该项目将进一步深入研究藏鸡独特的高原适应遗传特性，为培育新的抗TD的肉鸡品种提供新的思路。

中文关键词： 缺氧；胫骨生长板；血管内皮生长因子；藏鸡；肉鸡

英文摘要： Hypoxia increases the expressing levels of HIF-1α in mammalian and promote angiogenesis-related factors genreation,such as Hsp90, VEGF. Broilers tibial dyschondroplasia (TD) is due to the angiogenic disorders, Hypoxia is one of the key factor in the pathogenesis of Broiler TD.Our previous study showed Hsp90 expression level elevated in tibial growth plate in broiler TD, but it did not promote angiogenesis, the mechanism needs to be researched. Using Tibetan chicken as an comparison model, this project will compare the sequential difference expression of HIF-1α、Hsp90 and angiogenesis-related factors bFGF、VEGF and its receptor in growth plate between tibetan chicken and broiler chickens under hypoxic environmental conditions and thiram-induced tibial cartilage dysplasia; meanwhile, the mothed of cell culture is used to study chondrocytes differentiation、HIF-1α、Hsp90、bFGF、VEGF and its receptor expression characteristics from tibetan chicken and broiler under hypoxic conditions and thiram in medium.Based on these result the mechanism of different angiogenesis releated species in tibial growth plate is drown. The project will probe the advantage of genetic characteristics adapting plateau of tibetan chickens and provide new ideas for bring up new broiler breeds resistant TD.

英文关键词： hypoxia;tibial growth plate;vascular endothelial growth factor;tibetan chicken;broiler