不同产区太子参次生代谢物差异机制的iTRAQ定量蛋白质组学研究

项目名称： 不同产区太子参次生代谢物差异机制的iTRAQ定量蛋白质组学研究

项目编号： No.81473312

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 刘训红

作者单位： 南京中医药大学

项目金额： 75万元

中文摘要： 太子参为大宗中药材,具益气健脾、润肺生津之功能,疗效确切,临床用量大。然而,由于生态环境的不同,造成太子参有效成分的积累变异较大,质量参差不齐。如何在药材生产过程中，保证太子参的优质和质量均一已成为太子参生产过程中的关键问题。在太子参品质形成过程中，环境生态因子可通过一定途径影响太子参次生代谢物的合成与积累，然而不同生态环境太子参次生代谢物的积累差异机制如何尚未阐明，从而影响了太子参药材品质的提升工程。本项目采用植物代谢组学技术筛选和鉴定不同产区太子参次生代谢物差异的生物标记物，应用iTRAQ定量蛋白质组学方法筛选和分析不同产区太子参的差异表达蛋白质，通过对太子参代谢差异标记物与差异蛋白质的关联分析，寻找其目标差异蛋白质，结合其生物学功能，探讨不同产区太子参次生代谢物差异机制。从而为探索太子参药材品质形成过程提供基础资料，对研究中药材有效成分成因及品质形成机制具有示范性和指导。

中文关键词： 太子参；次生代谢物差异；代谢组学；蛋白质组学；iTRAQ

英文摘要： Pseudostellariae Radix, a kind of staple traditional Chinese medicine, has the functions of strengthening spleen, replenishing Qi, moistening lung and producing fluids. It has large clinical consumption for its positive effects. However, due to the difference of ecological environment, the variation of its effective components accumulation is big, and its quality is uneven. The key of manufacturing procedure of Pseudostellariae Radix is how to ensure its high and uniform quality. During the quality forming process of Pseudostellariae Radix, the environment ecology factors impact the synthesis and accumulation of its secondary metabolites through a certain way. But mechanism of the differences of the secondary metabolites of Pseudostellariae Radix growing in different ecological environments is unclear, which affects its quality improvement. This project will adopt the technology of plant metabolomics to filter and identify the biomarkers of metabolic differences of Pseudostellariae Radix from different production regions, and apply iTRAQ quantitative proteomics to screen and analyze the differential expression proteins of Pseudostellariae Radix from different regions. The mechanism of the differences of second metabolites in Pseudostellariae Radix will be discussed, through the correlation analysis of the biomarkers of metabolic differences and the differential expression proteins from Pseudostellariae Radix, and looking for the goal differential expression proteins, and combining with its biological function. Our research will provide the basic information for exploring the quality forming process, which will be demonstration and guidance to study effective constituents of Chinese materia medica and its quality formation mechanism.

英文关键词： Pseudostellariae Radix;Differences of Secondary Metabolites;Metabolomics;Proteomics;iTRAQ