应用三层一体化高仿生支架修复关节软骨缺损的实验研究

项目名称： 应用三层一体化高仿生支架修复关节软骨缺损的实验研究

项目编号： No.81472079

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 范卫民

作者单位： 南京医科大学

项目金额： 85万元

中文摘要： 目前，应用双层复合支架修复关节软骨缺损取得了良好的效果，较好地解决了组织工程软骨移植时固定困难、愈合慢、界面整合不良、移植物易脱落等问题。但人们又发现，此时组织工程软骨容易向软骨下骨过度生长，导致软骨及软骨下骨结构异常，且过厚的组织工程软骨会出现局部营养不良，从而导致组织工程软骨退变。理论上，在组织工程软骨与组织工程骨之间设计一个中间过渡层（隔离带）可以解决上述问题。我们拟使用丝素蛋白及β-磷酸三钙（β-TCP）作为支架材料，通过一体化构建法制作三层一体化高仿生支架。其中软骨部分为100%的丝素蛋白（孔径150～200μm，孔隙率90%以上）、骨部分为100%的β-TCP（孔径400～500μm，孔隙率70%以上）、中间过渡层为40%的丝素蛋白和60%β-TCP（孔径30～50μm，孔隙率75%以上）。体外构建组织工程骨软骨复合组织块，最后通过压配原理嵌入动物关节软骨缺损区修复软骨缺损。

中文关键词： 组织工程；支架；一体化；高仿生；关节软骨

英文摘要： Recently, application of biphasic monolithic scaffolds in cartilage defects repairing achieved good results. It could well resolve the problems during the tissue engineering scaffolds transplantation, such as difficulty in fixing, interface involution and slowly healed interface. And some disadvantages exist. For instance, this method could result in the downward overgrowth of tissue-engineered cartilage, leading to the structural abnormalities of cartilage and subchondral bone, which may ultimately result in abnormally stress transmission of cartilage and bone. In addition,tissue-engineered cartilages will be malnutrition in case they are too thick, which would ultimately cause the degeneration of cartilage and prevent the formation of tidemark region. Theoretically to design an intermediate layer between tissue-engineered cartilage and bone can solve the above problems. We aimed to manufacture a biomimetic three-layered scaffold with silk fibroin(100%) as a cartilage layer, silk fibroin(40%) and β-TCP(60%) as an intermediate layer, and β-TCP(100%) as a bony layer. Firstly, the scaffolds were seeded with autologous chondrocytes on the cartilage layer. And, before the scaffolds were transplanted into the defects, the bony layer were seeded with autologous BMSCs. At last, the scaffolds were embedded into articular defects by press-fitting principle in order to repair and regenerate osteochondral defects.

英文关键词： tissue engineering;scaffold;monolithic;biomimetic;cartilage