基于模型大鼠酒精性肝损伤CYP2E1基因调控机制的乌腺金丝桃组分配伍研究

项目名称： 基于模型大鼠酒精性肝损伤CYP2E1基因调控机制的乌腺金丝桃组分配伍研究

项目编号： No.81202638

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 胡晓阳

作者单位： 黑龙江中医药大学

项目金额： 24万元

中文摘要： 酒精性肝病的发病率呈逐年上升趋势。ALD的发病机制非常复杂。乙醇通过诱导CYP2E1基因致肝微粒体自由基产生增多，内源性抗氧化剂消耗增加及脂质过氧化反应增强，是酒精性肝损伤的主要原因之一。经过我们前期研究发现，乌腺金丝桃对急性酒精中毒小鼠血清ALT活性升高有抑制作用。本课题拟采用分析化学、系统生物学、药理学、血清药化学等多学科交叉综合手段，建立全面分离，重点分离相结合的乌腺金丝桃组分分离技术；以酒精性肝损伤模型大鼠的病理学检查、血清ALT和AST活性测定、肝脏中TG含量测定等研究乌腺金丝桃的复杂组效关系；采用组分配伍多靶点效应整合机制研究，观察乌腺金丝桃各种组分单独应用和配伍后对模型大鼠肝脏乙醇代谢酶系的影响和拮抗模型大鼠急性酒精性脂肪肝的分子机制，研究多组分配伍的整合调节规律，为现代中药组分配伍提供依据。

中文关键词： 乌腺金丝桃；酒精性肝损伤；治疗作用；作用机制；

英文摘要： The incidence of alcoholic liver disease is increasing year by year. Of ALD pathogenesis is very complex. Ethanol through the induction of CYP2E1 gene lead to liver microsomal free radical production increased consumption of endogenous antioxidants to increase and lipid peroxidation is one of the main reasons for alcoholic liver injury. After our preliminary studies found that black gland Hypericum inhibitory effect of elevated serum ALT activity of acute alcoholism. This project intends to analyze chemistry, systems biology, pharmacology, serum drug chemistry, interdisciplinary means, the establishment of a comprehensive separation, focus on separation of a combination of black glands of Hypericum component separation techniques; alcoholic liver injury model mouse pathology, serum ALT and AST activity was measured in liver TG content of the complexity of black gland Hypericum activity relationship; component compatibility of multi-target effects of integration mechanism the concept Chawu glands of Hypericum each component alone and compatibility impact and antagonistic rat model of acute alcoholic fatty liver of the molecular mechanisms of ethanol metabolic enzymes in rat liver model to study the multi-component compatibility integration regulating law, to provide compatibility for modern traditional Chinese me

英文关键词： Hypericum attenuatum；Ald；Therapeutic effect；Mechanism of action；