樱桃耐涝相关基因的分离与功能解析

项目名称： 樱桃耐涝相关基因的分离与功能解析

项目编号： No.31301746

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 生利霞

作者单位： 扬州大学

项目金额： 23万元

中文摘要： 樱桃是我国大面积栽培的高档果品，但其根系浅，对环境变化反应敏感，常因淹涝造成严重的经济损失。采取现代分子生物学手段，改良樱桃的耐涝性，具有十分重要的理论意义和应用价值。本项目拟从与植物抗逆性相关的ERFs和CIPKs基因家族入手，以与这两个基因家族成员紧密关联、并在响应淹涝胁迫中发挥重要作用的碳水化合物代谢为主线，在前期已筛选获得耐/敏涝樱桃特异资源的基础上，克隆樱桃耐涝特异ERFs和CIPKs基因家族成员，分析其时空表达模式，利用RNAi和基因超量表达技术验证其功能，确定调控樱桃耐涝性的关键基因，并通过分析樱桃特异耐涝基因与碳水化合物代谢和根系发生相关基因家族成员在淹涝胁迫下表达情况的一致性和相关性，探明樱桃应答淹涝胁迫的响应途径和调节机制，阐明樱桃种质耐涝能力强弱差异的分子机理。研究结果将为樱桃及其它果树耐涝性的分子遗传改良奠定基础，并有望获得转基因耐涝樱桃新种质。

中文关键词： 樱桃；耐涝基因；时空表达；功能验证；耐涝机制

英文摘要： Cherry is a kind of high-grade fruit widely cultivated in China. Although its root system is very shallow, it is sensitive to environmental changes. Serious economic losses often results from the destruction of cherry farms due to floods. Molecular biology methods for improving waterlogging-tolerance of cherries will have very important theoretical and practical significance. This project plans to start with the ERFs and CIPKs gene families relevant to plant stress resistance. Carbohydrate metabolism, which is closely related to the members of these two gene families and plays an essential role in the response to waterlogging stress, will be taken as the core of this study. Based on the waterlogging resistance of several domestic and overseas cherry species evaluated at an early stage. Specific species with a strong waterlogging resistance will be identified. Family members of specific waterlogging-tolerant ERFs and CIPKs genes in cherry shall be cloned to analyze their expression patterns in time and space. Their functions will be verified by the gene silencing and overexpression technology to determine the key genes for controlling the waterlogging resistance. These effects of specific waterlogging genes, the carbohydrate metabolism, as well as the consistency and correlation of the expression of root-developm

英文关键词： Cherry；Waterlogging-tolerance gene；Temporal and spatial expression；Functional verification；Waterlogging-tolerance mechanism