科研与周边环境中低剂量纳米材料的长期暴露水平及其毒理学效应研究

项目名称： 科研与周边环境中低剂量纳米材料的长期暴露水平及其毒理学效应研究

项目编号： No.21277037

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 环境科学、安全科学

项目作者： 赵宇亮

作者单位： 国家纳米科学中心

项目金额： 80万元

中文摘要： 尽管纳米材料的环境健康效应已经引起学术界的高度重视，然而，针对实验室与周边环境中低剂量长期暴露的纳米材料的毒理学效应却鲜有研究，已成为亟待探索的重要前沿科学问题。单原子层厚度的石墨烯是人类目前拥有的结构和功能都最为特殊的纳米材料（2010年诺贝尔奖），其长期低剂量暴露下的毒理学效应研究尚是空白。本项目首先对石墨烯合成与研究的实验室环境中的石墨烯暴露水平进行监测和分析，确定实际暴露水平。建立定量方法，利用线虫模型定量研究低剂量长期暴露下的抗性、生长发育水平、应激能力、生殖和遗传等功能的变化。建立多种转基因及基因突变体（重点针对应激抗性基因）线虫模型，探索石墨烯暴露对体内化学生物微环境不同的易感机体产生的负面生物效应的分子机制。获得高水平原创性基础成果的同时，探索针对科研群体的暴露限量值（OELs），为国家建立相关的安全防护规范和标准提供科学依据。

中文关键词： 科研环境；纳米安全；颗粒物释放与监测；核分析技术；纳米毒理学

英文摘要： The environmental health and safety issues of nanotechnology are highly concerned recently. However, little is known about the long-term toxicity of exposure to nanomaterials in the laboratory and surrounding environment. Graphene, a single-atom-thick sheet of graphite, is a novel nanomaterial of unique functions and structures (2010 Nobel Prize in Physics). However, the long-term toxicity upon low levels exposure of graphene is unknown. To this end, we firstly monitor the exposure of graphene-based nanomaterials during synthesis and research processes, to obtain the data of exposure levels at the laboratory and surrounding environment. Then, we will establish the methodology on quantificational assessment of the long-term toxicity under real exposure levels using Caenorhabditis elegans (C. elegans). The lifespan, reproduction, behavior, oxidative stress and thermotolerance will be tested to evaluate the long-term nanosafety. The susceptibility tests will be performed using transgenic and mutant C. elegans under both normal and stress conditions. The molecular mechanisms of the susceptiblility on graphene-based nanomaterials exposure will be elucidated. In addition to generate the fundamental knowledge on toxicity properties of graphene-based nanomaterials at the low-level and long term exposure, we also aim to

英文关键词： laboratory environments；nanosafety；particle release and monitor；nuclear analytical techniques；nanotoxicology