基于稳定动脉粥样硬化斑块及缺血心肌新生血管的芪参益气方防治冠心病的机制研究

项目名称： 基于稳定动脉粥样硬化斑块及缺血心肌新生血管的芪参益气方防治冠心病的机制研究

项目编号： No.81202943

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 张密霞

作者单位： 天津中医药大学

项目金额： 23万元

中文摘要： 动脉粥样硬化（AS）斑块不稳定是导致急性心血管事件的主要原因，抑制斑块内新生血管可以增强斑块稳定性，但对缺血组织的代偿性血管新生也存在抑制作用。而斑块不稳定与其新生血管结构不成熟密切相关，与斑块相关的心肌缺血区也存在血管新生，因此稳定斑块内新生血管，促进缺血心肌血管新生和成熟，更符合防治AS的临床特点。芪参益气方可抑制和稳定AS斑块并可改善缺血心肌血流灌注。故本研究从稳定新生血管入手，复制猪AS兼心肌缺血再灌注模型，检测芪参益气方对斑块稳定性、缺血心肌、斑块及心肌内血管新生及成熟的影响；并通过血管成熟相关VEGF、Ang1-Tie2通路、TGF-β通路、bFGF/PDGF-BB/PDGFR-α、β通路探讨其稳定新生血管的机制；体外培养主动脉血管段，检测该方对血管新生、稳定及消退的影响，并加入上述通路抑制剂，进一步验证芪参益气方稳定新生血管的作用。为中医药治疗AS提供科学依据。

中文关键词： 芪参益气滴丸；心肌梗死；动脉粥样硬化；血管新生；

英文摘要： Atherosclerosis ( AS ) plaque instability is the main reason leading to acute cardiovascular events, inhibition of plaque neovascularization can enhance plaque stability, but it also inhibit the compensatory angiogenesis of ischemic tissue. And instability plaque and neovascular structures of immature closely related. The myocardial ischemia area associated with plaques also has angiogenesis. Thus stable plaque neovascularization and promote neovascularization of myocardial ischemia in the same time maybe in line with the AS clinical characteristics. Qishen Yiqi formula can restrain and stabilize AS plaques and can improve the ischemic myocardial blood flow perfusion. So we research the effection of Qishen Yiqi formula on atherosclerosis basedon the stability of neovascularization. In this study ,we use swine AS and myocardial ischemia reperfusion model to detect the effection of Qishen Yiqi Decoction on plaque stability, myocardial ischemia, angiogenesis of plaque and myocardial and thier maturation. Then measure the vessel maturation correlates pathway, including VEGF, Ang1-Tie2 pathway, TGF beta pathway, bFGF / PDGF-BB / PDGFRα、β to looking for the mechanism. We also cultured aortic segment in vitro, detect the effection of Qishen Yiqi formula on angiogenesis, stabilization and regression. Then add inhibito

英文关键词： qinshenyiqi pills；myocardial infarction；atherosclerosis；Angiogenesis；